Abstract
Synchrotron-based X-ray fluorescence (XRF) is allowing substantial advances in several disciplines of plant science by allowing the in situ examination of elements within plant tissues. Continual improvements in detector speed, sensitivity, and resolution are increasing the diversity of questions that can be addressed using this technique, including the in situ analysis of elements (such as nutrients or toxicants) within fresh and hydrated tissues. Here, we describe the general principles for designing and conducting experiments for the examination of elemental distributions in plant material using micro-XRF.
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Acknowledgements
The method described in this chapter is based on work carried out at the X-ray Fluorescence Microscopy (XFM) beamline at the Australian Synchrotron. We would like to acknowledge the XFM beamline scientists David Paterson and Daryl Howard, and the GeoPIXE image analysis software developer Chris Ryan (CSIRO) for their expertise and support. We also thank Dr Simon A. James (Australian Synchrotron) for proof reading and commenting on the manuscript.
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Donner, E., de Jonge, M.D., Kopittke, P.M., Lombi, E. (2013). Mapping Element Distributions in Plant Tissues Using Synchrotron X-ray Fluorescence Techniques. In: Maathuis, F. (eds) Plant Mineral Nutrients. Methods in Molecular Biology, vol 953. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-152-3_9
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DOI: https://doi.org/10.1007/978-1-62703-152-3_9
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