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Use of Boronic Acid Nanoparticles in Glycoprotein Enrichment

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Mass Spectrometry of Glycoproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 951))

Abstract

Glyco-specific enrichment methods for mass spectrometry pretreatment are invaluable for the detection of low abundant glycoproteins or glycopeptides. For example, boronic acid can specifically interact with glycans in nonaqueous or basic aqueous solutions. Here, we describe a glyco-specific enrichment method which uses a boronic acid-functionalized “core-satellite” composite nanoparticle to isolate glycoproteins or glycopeptides from complex biological samples. Furthermore, we also demonstrate detection limit improvements and show how to evaluate the percent recovery from the glycoprotein or glycopeptide enrichment process via SDS-PAGE and 16O/18O labeling strategies.

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Acknowledgments

This work was supported by National Science and Technology Key Project of China (2007CB914100 and 2009CB825607), National Natural Science Foundation of China (31070732 and 21025519), Shanghai Projects (Shuguang, Eastern Scholar and B109).

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Authors and Affiliations

  1. Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, China

    Yawei Xu, Lijuan Zhang & Haojie Lu

Authors
  1. Yawei Xu
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  2. Lijuan Zhang
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  3. Haojie Lu
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Corresponding author

Correspondence to Haojie Lu .

Editor information

Editors and Affiliations

  1. Medical Center, Department of Biochemistry, University of Texas Southwestern, Harry Hines Blvd. 5323, Dallas, 75390-9038, Texas, USA

    Jennifer J. Kohler

  2. Medical Center, Dallas, University of Texas Southwestern, Harry Hines Blvd. 5323, Dallas, 75390-9185, Texas, USA

    Steven M. Patrie

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Xu, Y., Zhang, L., Lu, H. (2013). Use of Boronic Acid Nanoparticles in Glycoprotein Enrichment. In: Kohler, J., Patrie, S. (eds) Mass Spectrometry of Glycoproteins. Methods in Molecular Biology, vol 951. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-146-2_4

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  • DOI: https://doi.org/10.1007/978-1-62703-146-2_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-145-5

  • Online ISBN: 978-1-62703-146-2

  • eBook Packages: Springer Protocols

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