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Microfluidic Diagnostics pp 387–401Cite as

Multilayer Microfluidic Poly(Ethylene Glycol) Diacrylate Hydrogels

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 949))

Abstract

Development of robust, in vivo like tissues in vitro holds the potential to create regenerative medicine-based therapeutics, provide more physiologically significant preclinical models and supply a pharmacological and toxicological screening platform that reflects in vivo systems in both complexity and function. This protocol describes a simple, robust, multilayer replica molding technique in which poly(dimethylsiloxane) (PDMS) and poly(ethylene glycol) diacrylate (PEGDA) are serially replica molded to develop microfluidic PEGDA hydrogel networks embedded within independently fabricated PDMS housings, using a combination of soft and photo-lithography. This work has direct applications toward the development of robust, complex, cell-laden hydrogels for in vitro diagnostics and regenerative medicine applications.

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Acknowledgments

The author would like to thank Professor Lisa Biswal and her research group, especially Gautam Kini, for technical assistance and Melissa McHale for her helpful insight proofreading and improving this chapter. This work was supported by the NIH Biotechnology Training Grant (T32 GM008362-18) and the NIH Quantum Grant (1 P20 EB007076 01).

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Authors and Affiliations

  1. Department of Bioengineering, MS-142, BRC, Rice University, Houston, TX, USA

    Michael P. Cuchiara & Jennifer L. West

Authors
  1. Michael P. Cuchiara
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  2. Jennifer L. West
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Corresponding author

Correspondence to Jennifer L. West .

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Editors and Affiliations

  1. Key Laboratory for Organic Electronics &, Information Displays (KLOEID), Nanjing Univ. of Posts&Telecommunication, Jia Sheng Hua Yuan, No.2 Building Room 201, Nanjing, 210013, Jiangsu, China, People's Republic

    Gareth Jenkins

  2. Scientific & Medical Communications Cons, avenue Paul Santy 1C, Ecully, 69130, France

    Colin D. Mansfield

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Cuchiara, M.P., West, J.L. (2013). Multilayer Microfluidic Poly(Ethylene Glycol) Diacrylate Hydrogels. In: Jenkins, G., Mansfield, C. (eds) Microfluidic Diagnostics. Methods in Molecular Biology, vol 949. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-134-9_24

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  • DOI: https://doi.org/10.1007/978-1-62703-134-9_24

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-133-2

  • Online ISBN: 978-1-62703-134-9

  • eBook Packages: Springer Protocols

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