Abstract
Head and neck reconstruction transplants often require a bony structure but also tissue for the intraoral lining. This is why oral keratinocytes and osteoblast-like cells are essential cell types for combined tissue engineered transplants for defects in the field of craniomaxillofacial surgery. Therefore, we isolated oral keratinocytes and osteoblast-like cells from human tissue samples and cocultivated both cell types on the same carrier. Cell proliferation and morphological analysis showed that the contemporaneous cultivation of human oral keratinocytes and human osteoblast-like cells is possible.
The successful in vitro cocultivation of hard and soft tissue derived cells on the same carrier will be an important advancement for developing hard and soft tissue reconstruction therapies especially in the oral cavity.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Wan DC, Nacamuli RP, Longaker MT (2006) Craniofacial bone tissue engineering. Dent Clin North Am 50:175–190, vii
Lendeckel S, Jodicke A, Christophis P et al (2004) Autologous stem cells (adipose) and fibrin glue used to treat widespread traumatic calvarial defects: case report. J Craniomaxillofac Surg 32:370–373
Schleicher I, Parker A, Leavesley D et al (2005) Surface modification by complexes of vitronectin and growth factors for serum-free culture of human osteoblasts. Tissue Eng 11:1688–1698
Schmelzeisen R, Schimming R, Sittinger M (2003) Making bone: implant insertion into tissue-engineered bone for maxillary sinus floor augmentation – a preliminary report. J Craniomaxillofac Surg 31:34–39
Terheyden H, Warnke P, Dunsche A et al (2001) Mandibular reconstruction with prefabricated vascularized bone grafts using reccombinant human osteogenic protein-1: an experimental study in miniature pigs. Part II: transplantation. Int J Oral Maxillofac Surg 30:469–478
Lauer G, Otten JE (1997) Cultivation of gingival keratinocytes on permeable membranes: simulation of the function of mouth cavity epithelium. Mund Kiefer Gesichtschir 1:35–38
Kedjarune U, Pongprerachok S, Arpornmaekl-ong P et al (1997) Culturing primary human gingival epithelial cells: comparison of two isolation techniques. J Craniomaxillofac Surg 29:224–231
Jonsson KB, Frost A, Nilsson O et al (1999) Three isolation techniques for primary culture of human osteoblast-like cells: a comparison. Acta Orthop Scand 70:365–373
Voegele TJ, Voegele-Kadletz M, Esposito V et al (2000) The effect of different isolation techniques on human osteoblast-like cell growth. Anticancer Res 20:3575–3581
Glaum R (2008) Tissue Engineering von Composite Grafts: Cokultivierung von Gingivakeratinozyten und Osteoblasten auf Polycarbonat- und Kollagenmembranen. VVB Laufersweiler Verlag, Giessen
Sendic M (2005) Wachstumsverhalten und gegenseitige Beeinflussung von Gingivakeratino-zyten und Osteoblasten auf Kollagen. Inaugural-Dissertation zur Erlangung des Zahnmedizinischen Doktorgrades der Medizinischen Fakultät der Albert-Ludwigs-Universität Freiburg i Br
Glaum R, Wiedmann-Al-Ahmad M, Huebner U et al (2010) Tissue engineering of composite grafts: cocultivation of human oral keratinocytes and human osteoblast-like cells on laminin-coated polycarbonate membranes and equine collagen membranes under different culture conditions. J Biomed Mater Res A 93:704–715
Otto F (2004) Vergleichende Untersuchungen des Wachstums humaner Osteoblasten nach Kultivierung im Brutschrank und in der Perfusionskammer. Inaugural-Dissertation zur Erlangung des Medizinischen Doktorgrades der Medizinischen Fakultät der Albert-Ludwigs-Universität Freiburg i Br, Freiburg
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Glaum, R., Wiedmann-Al-Ahmad, M. (2013). Cocultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells. In: Helgason, C., Miller, C. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology, vol 946. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-128-8_27
Download citation
DOI: https://doi.org/10.1007/978-1-62703-128-8_27
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-127-1
Online ISBN: 978-1-62703-128-8
eBook Packages: Springer Protocols