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Isolation and Characterization of Mouse Side Population Cells

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Basic Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 946))

Abstract

The side population (SP) is a subpopulation of mouse bone marrow cells highly enriched for hematopoietic stem cell activity. The SP is identified using flow cytometry as a minor population that efficiently effluxes the DNA-binding dye Hoechst 33342 relative to the rest of the bone marrow. Phenotypic and functionally analysis has established SP cells as highly phenotypically homogeneous and functional active. In this chapter we describe a detailed protocol for the purification of murine bone marrow SP cells based on Hoechst dye efflux in combination with the presence of HSC surface markers.

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Acknowledgments

The authors are supported by grants from the NIH, the Ellison Foundation, and the American Heart Association. M.J. was supported by University of Science and Technology though UST Post-Doc Research Program. G.A.C. is a scholar of the American Society of Hematology.

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Authors and Affiliations

  1. The Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX, USA

    Aysegul V. Ergen, Mira Jeong, Kuanyin K. Lin & Grant A. Challen

  2. University of Science and Technology, Daejeon, South Korea

    Mira Jeong

  3. Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, TX, USA

    Margaret A. Goodell

Authors
  1. Aysegul V. Ergen
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  2. Mira Jeong
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  3. Kuanyin K. Lin
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  4. Grant A. Challen
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  5. Margaret A. Goodell
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Corresponding author

Correspondence to Margaret A. Goodell .

Editor information

Editors and Affiliations

  1. , Experimental Therapeutics, BC Cancer Agency, West 10th Ave. 675, Vancouver, V5Z 1L3, British Columbia, Canada

    Cheryl D. Helgason

  2. STEMCELL Technologies, Inc., W. Seventh Avenue 570, Vancouver, V5Z 1B3, British Columbia, Canada

    Cindy L. Miller

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Ergen, A.V., Jeong, M., Lin, K.K., Challen, G.A., Goodell, M.A. (2013). Isolation and Characterization of Mouse Side Population Cells. In: Helgason, C., Miller, C. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology, vol 946. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-128-8_10

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  • DOI: https://doi.org/10.1007/978-1-62703-128-8_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-127-1

  • Online ISBN: 978-1-62703-128-8

  • eBook Packages: Springer Protocols

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