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Fungal Secondary Metabolism pp 47–58Cite as

Media and Growth Conditions for Induction of Secondary Metabolite Production

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 944))

Abstract

Growth media and incubation conditions have a very strong influence of secondary metabolite production. There is no consensus on which media are the optimal for metabolite production, but a series of useful and effective media and incubation conditions have been listed here. Chemically well-defined media are suited for biochemical studies, but in order to get chemical diversity expressed in filamentous fungi, sources rich in amino acids, vitamins, and trace metals have to be added, such as yeast extract and oatmeal. A battery of solid agar media is recommended for exploration of chemical diversity as agar plug samples are easily analyzed to get an optimal representation of the qualitative secondary metabolome. Standard incubation for a week at 25°C in darkness is recommended, but optimal conditions have to be modified depending on the ecology and physiology of different filamentous fungi.

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Acknowledgements

The project was supported by the Danish Research Council for Technology and Production.

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  1. Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Kongens Lyngby, Denmark

    Jens C. Frisvad

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  1. Jens C. Frisvad
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Correspondence to Jens C. Frisvad .

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  1. , Dept. of Medical Microbiology & Immunolo, University of Wisconsin-Madison, Linden Drive 1550, Madison, 53706, Wisconsin, USA

    Nancy P. Keller

  2. , Dept. of Molecular Biology & Biotechnolo, University of Sheffield, Firth Court, Sheffield, S10 2TN, United Kingdom

    Geoffrey Turner

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Frisvad, J.C. (2012). Media and Growth Conditions for Induction of Secondary Metabolite Production. In: Keller, N., Turner, G. (eds) Fungal Secondary Metabolism. Methods in Molecular Biology, vol 944. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-122-6_3

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  • DOI: https://doi.org/10.1007/978-1-62703-122-6_3

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