Abstract
With the completion of genomes of various Aspergillus species, large-scale genome-wide expression studies can be carried out. Genomics, however, is more powerful and efficient when combined with genetics. A multi-copy-based gain-of-function screen is a complementary method to loss-of-function genetic screen and can identify novel genes that may not be easily identifiable through loss-of-function-type screens. Particularly, gain-of-function genetic screens would identify novel activators or repressors of fungal development and secondary metabolism. Here, we describe a working protocol for the identification of novel regulators in Aspergillus nidulans.
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Acknowledgments
The authors express special thanks to Drs. Nak-Jung Kwon and Ni Min for their technical assistance. This work was supported by the National Science Foundation (IOS-0950850), USDA CSREES Hatch (WIS04667), and UW Food Research Institute grants to JHY. HSP was in part supported by the UW FRI Foster Award.
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Park, H.S., Yu, JH. (2012). Multi-Copy Genetic Screen in Aspergillus nidulans . In: Keller, N., Turner, G. (eds) Fungal Secondary Metabolism. Methods in Molecular Biology, vol 944. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-122-6_13
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DOI: https://doi.org/10.1007/978-1-62703-122-6_13
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-121-9
Online ISBN: 978-1-62703-122-6
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