Abstract
Plasmid vector construction is an essential step for molecular microbiology yet often a time-consuming process. Manipulation of the fungal genome to express genes to activate secondary metabolite production often requires creation of plasmid constructs in a reiterative fashion. Here we introduce a modified Quick-change site-directed mutagenesis method that allows for rapid and accurate construction of fungal transformation vectors.
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Acknowledgment
The project was supported by PO1GM084077 from the National Institute of General Medical Sciences to N.P.K.
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Bok, J.W., Keller, N.P. (2012). Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis. In: Keller, N., Turner, G. (eds) Fungal Secondary Metabolism. Methods in Molecular Biology, vol 944. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-122-6_11
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DOI: https://doi.org/10.1007/978-1-62703-122-6_11
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-122-6
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