Abstract
Milligram quantities of RNA are commonly synthesized by in vitro transcription from a DNA template with T7 RNA polymerase. However, the run-off transcription method results in heterogeneity at the RNA 3′-terminus. RNA purification requires single-nucleotide resolution to separate the transcript of the correct length from the aborted or add-on transcripts that are usually present in comparable amounts. Here, we describe an RNA preparation method that uses a trans-acting DNAzyme and sequence-specific affinity column chromatography. This purification method is simple, fast, and suited for high throughput.
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Cheong, HK., Hwang, E., Cheong, C. (2013). Rapid Preparation of RNA Samples Using DNA-Affinity Chromatography and DNAzyme Methods. In: Conn, G. (eds) Recombinant and In Vitro RNA Synthesis. Methods in Molecular Biology, vol 941. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-113-4_9
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DOI: https://doi.org/10.1007/978-1-62703-113-4_9
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-112-7
Online ISBN: 978-1-62703-113-4
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