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Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-vacuum Gene Gun (HandyGun)

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Biolistic DNA Delivery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 940))

Abstract

Non-vacuum gene guns such as HandyGun are flexible tools for bombardment of targets of varying size. Construction of HandyGun is simpler and cheaper than vacuum gene guns and will be described here. The conditions for maximal transient transformation efficiency of plant cells with plasmid DNA using HandyGun will be provided.

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References

  1. Gal-On A et al (1997) Simple hand-held devices for the efficient infection of plants with viral-encoding constructs by particle bombardment. J Virol Methods 64:103–110

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  2. Gaba V, Gal-On A (2005) Inoculation of plants using bombardment. In: Simon A, Kowalik T, Quarles J (eds) Current protocols in microbiology. Wiley Interscience, New York, pp 16B.3.1–16B.3.14

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  3. Gray DJ et al (1994) Simplified construction and performance of a device for particle bombardment. Plant Cell Tissue Organ Cult 37:179–184

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  4. Sikorskaite S et al (2010) HandyGun: an improved custom-designed, non-vacuum gene gun suitable for virus inoculation. J Virol Methods 165:320–324

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Acknowledgments

The work was supported financially by the Academy of Finland (grants 118766 and 1134759 to J.P.T.V.).

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Authors and Affiliations

  1. Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland

    Anssi L. Vuorinen, Sidona Sikorskaite & Jari P. T. Valkonen

  2. The Instrument Center, University of Helsinki, Helsinki, Finland

    Arto Nieminen

  3. Department of Plant Pathology and Weed Science, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel

    Victor Gaba

Authors
  1. Anssi L. Vuorinen
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  2. Arto Nieminen
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  3. Victor Gaba
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  4. Sidona Sikorskaite
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  5. Jari P. T. Valkonen
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Corresponding author

Correspondence to Jari P. T. Valkonen .

Editor information

Editors and Affiliations

  1. Ganzimmun Diagnostics AG, Hans-Böckler-Str. 109, Mainz, 55128, Germany

    Stephan Sudowe

  2. University Medical Center, Department of Dermatology, Johannes Gutenberg-University, Obere Zahlbacher Str. 63, Mainz, 55131, Germany

    Angelika B. Reske-Kunz

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Vuorinen, A.L., Nieminen, A., Gaba, V., Sikorskaite, S., Valkonen, J.P.T. (2013). Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-vacuum Gene Gun (HandyGun). In: Sudowe, S., Reske-Kunz, A. (eds) Biolistic DNA Delivery. Methods in Molecular Biology, vol 940. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-110-3_4

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  • DOI: https://doi.org/10.1007/978-1-62703-110-3_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-109-7

  • Online ISBN: 978-1-62703-110-3

  • eBook Packages: Springer Protocols

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