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Measurement of Cytosolic-Free Ca2+ in Plant Tissue

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Calcium Signaling Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 937))

Abstract

A range of techniques have been used to measure the concentration of cytosolic-free Ca2+ ([Ca2+]cyt) in plant cells. Fluorescent Ca2+-sensitive indicators have been used extensively to measure plant [Ca2+]cyt and a number of techniques are available for loading these into plant cells. Here we describe a method for measuring [Ca2+]cyt in the guard cells of the model plant species Commelina communis by ratio photometry and imaging techniques using the ratiometric fluorescent Ca2+-sensitive indicator fura-2.

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Acknowledgments

The authors thank the European Community, The Royal Society, the Natural Environment Research Council (UK), the Biotechnology and Biological Sciences Research Council (UK), Science Foundation Ireland (Republic of Ireland), and the Irish Research Council for Science, Engineering and Technology (Republic of Ireland) for funding.

Author information

Authors and Affiliations

  1. Lancaster Environment Centre, Lancaster University, Lancaster, UK

    Martin R. McAinsh

  2. School of Biology and Environmental Science, University College Dublin, Dublin, Ireland

    Carl K.-Y. Ng

Authors
  1. Martin R. McAinsh
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  2. Carl K.-Y. Ng
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Corresponding author

Correspondence to Martin R. McAinsh .

Editor information

Editors and Affiliations

  1. , Department of Cardiovascular Sciences, Leicester Royal Infirmary, Infirmary Square, Leicester, LE1 5WW, Leicestershire, United Kingdom

    David G. Lambert

  2. , Department of Cardiovascular Sciences, Leicester Royal Infirmary, Infirmary Square, Leicester, LE1 5WW, Leicestershire, United Kingdom

    Richard D. Rainbow

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McAinsh, M.R., Ng, C.KY. (2013). Measurement of Cytosolic-Free Ca2+ in Plant Tissue. In: Lambert, D., Rainbow, R. (eds) Calcium Signaling Protocols. Methods in Molecular Biology, vol 937. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-086-1_20

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  • DOI: https://doi.org/10.1007/978-1-62703-086-1_20

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-085-4

  • Online ISBN: 978-1-62703-086-1

  • eBook Packages: Springer Protocols

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