Abstract
Calcium recording from whole heart is an important technique to investigate role of calcium in cardiac arrhythmias. Intracellular calcium can be recorded from multiple locations using imaging devices and organic dyes or genetic probe (Tallini et al. PNAS 103(12):4753–4758, 2006) from whole heart. Here, we describe the optical apparatus and the method to record intracellular calcium transients.
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References
Wongcharoen W et al (2007) Aging increases pulmonary veins arrhythmogenesis and susceptibility to calcium regulation agents. Heart Rhythm 4(10):1338–1349
Ono N et al (2007) Spontaneous atrial fibrillation initiated by triggered activity near the pulmonary veins in aged rats subjected to glycolytic inhibition. Am J Physiol Heart Circ Physiol 292(1):H639–H648
Omichi C et al (2004) Intracellular Ca dynamics in ventricular fibrillation. Am J Physiol Heart Circ Physiol 286(5):H1836–H1844
Chudin E et al (1999) Intracellular Ca(2+) dynamics and the stability of ventricular tachycardia. Biophys J 77(6):2930–2941
Hoeker GS et al (2009) Spontaneous calcium release in tissue from the failing canine heart. Am J Physiol Heart Circ Physiol 297(4):H1235–H1242
Curran J et al (2010) Spontaneous Ca waves in ventricular myocytes from failing hearts depend on Ca(2+)-calmodulin-dependent protein kinase II. J Mol Cell Cardiol 49(1):25–32
Belevych AE et al (2009) Redox modification of ryanodine receptors underlies calcium alternans in a canine model of sudden cardiac death. Cardiovasc Res 84(3):387–395
Chou CC et al (2007) Remodelling of action potential and intracellular calcium cycling dynamics during subacute myocardial infarction promotes ventricular arrhythmias in Langendorff-perfused rabbit hearts. J Physiol 580(Pt. 3):895–906
Clusin WT (2008) Mechanisms of calcium transient and action potential alternans in cardiac cells and tissues. Am J Physiol Heart Circ Physiol 294(1):H1–H10
Szabo B, Kovacs T, Lazzara R (1995) Role of calcium loading in early afterdepolarizations generated by Cs+ in canine and guinea pig Purkinje fibers. J Cardiovasc Electrophysiol 6(10 Pt 1):796–812
Verduyn SC et al (1995) The effect of flunarizine and ryanodine on acquired torsades de pointes arrhythmias in the intact canine heart. J Cardiovasc Electrophysiol 6(3):189–200
Choi BR, Burton F, Salama G (2002) Cytosolic Ca2+ triggers early afterdepolarizations and Torsade de Pointes in rabbit hearts with type 2 long QT syndrome. J Physiol 543(Pt 2):615–631
Volders PG et al (1997) Similarities between early and delayed afterdepolarizations induced by isoproterenol in canine ventricular myocytes. Cardiovasc Res 34(2):348–359
Saitoh H, Bailey JC, Surawicz B (1989) Action potential duration alternans in dog Purkinje and ventricular muscle fibers. Further evidence in support of two different mechanisms. Circulation 80(5):1421–1431
Shimizu W, Antzelevitch C (1999) Cellular and ionic basis for T-wave alternans under long-QT conditions. Circulation 99(11):1499–1507
Sato D et al (2006) Spatially discordant alternans in cardiac tissue: role of calcium cycling. Circ Res 99(5):520–527
Hayashi H et al (2007) Dynamic origin of spatially discordant alternans in cardiac tissue. Biophys J 92(2):448–460
Bao M et al (2007) Abnormal intracellular calcium handling underlying T-wave alternans and its hysteresis. Cardiology 108(3):147–156
Sato D et al (2007) Inferring the cellular origin of voltage and calcium alternans from the spatial scales of phase reversal during discordant alternans. Biophys J 92(4):L33–L35
Pruvot EJ et al (2004) Role of calcium cycling versus restitution in the mechanism of repolarization alternans. Circ Res 94(8):1083–1090
Lee HC et al (1987) Cytosolic calcium transients from the beating mammalian heart. Proc Natl Acad Sci USA 84(21):7793–7797
Wu Y, Clusin WT (1997) Calcium transient alternans in blood-perfused ischemic hearts: observations with fluorescent indicator fura red. Am J Physiol 273(5 Pt 2):H2161–H2169
Choi BR, Liu T, Salama G (2006) Calcium transients modulate action potential repolarizations in ventricular fibrillation. Conf Proc IEEE Eng Med Biol Soc 1:2264–2267
Choi BR, Salama G (2000) Simultaneous maps of optical action potentials and calcium transients in guinea-pig hearts: mechanisms underlying concordant alternans. J Physiol 529(Pt 1):171–188
Del Nido PJ et al (1998) Fluorescence measurement of calcium transients in perfused rabbit heart using rhod 2. Am J Physiol 274(2 Pt 2):H728–H741
Choi BR, Salama G (2000) Simultaneous maps of optical action potentials and calcium transients in guinea-pig hearts: mechanisms underlying concordant alternans. J Physiol 529(Pt 1):171–188
duBell WH et al (1991) The cytosolic calcium transient modulates the action potential of rat ventricular myocytes. J Physiol 436:347–369
Schlotthauer K, Bers DM (2000) Sarcoplasmic reticulum Ca(2+) release causes myocyte depolarization. Underlying mechanism and threshold for triggered action potentials. Circ Res 87(9):774–780
Acknowledgement
This work was supported by NIH grant R01 HL096669-01 to BRC.
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Choi, BR. (2013). Calcium Measurements from Whole Heart Using Rhod-2. In: Lambert, D., Rainbow, R. (eds) Calcium Signaling Protocols. Methods in Molecular Biology, vol 937. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-086-1_13
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DOI: https://doi.org/10.1007/978-1-62703-086-1_13
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Publisher Name: Humana Press, Totowa, NJ
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