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MicroRNA Expression Profiling During Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells

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MicroRNA Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 936))

Abstract

The Mouse P19 cell line was derived from an embryonal carcinoma. The pluripotent P19 cells can be induced to differentiate into neuronal and glial cells. Here, we describe an miRNA microarray method to monitor the miRNA expression profiles during the course of P19 cells neuronal differentiation. The results indicated that there were significant differences between the miRNA expression in P19 EC cells and the resultant differentiated neural stem cells.

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References

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Authors and Affiliations

  1. School of Life Sciences, Shanghai University, Shanghai, China

    Botao Zhao & Youxin Jin

  2. State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

    Bing Huang & Wei Li

Authors
  1. Botao Zhao
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  2. Bing Huang
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  3. Wei Li
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  4. Youxin Jin
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Corresponding author

Correspondence to Youxin Jin .

Editor information

Editors and Affiliations

  1. Keck School of Medicine, Dept. of Cell and Neurobiology, University of Southern California, San Pablo St. 1333, Los Angeles, 90033, California, USA

    Shao-Yao Ying

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Zhao, B., Huang, B., Li, W., Jin, Y. (2013). MicroRNA Expression Profiling During Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells. In: Ying, SY. (eds) MicroRNA Protocols. Methods in Molecular Biology, vol 936. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-083-0_9

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  • DOI: https://doi.org/10.1007/978-1-62703-083-0_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-082-3

  • Online ISBN: 978-1-62703-083-0

  • eBook Packages: Springer Protocols

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