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MicroRNA Expression During Neuronal Differentiation of Human Teratocarcinoma NTera2D1 and Mouse Embryonic Carcinoma P19 Cells

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MicroRNA Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 936))

Abstract

MicroRNAs (miRNAs) are 21–23-nucleotide-long small noncoding RNAs, function as mediators in gene silencing, and play essential roles in gene regulation in development, differentiation, and proliferation. Hundreds of miRNAs have been found, and tissue-specific or organ-specific expression of miRNAs has been detected. Here, I describe procedures for detection of miRNAs in the course of neuronal differentiation of human teratocarcinoma NTera2D1 and mouse embryonic carcinoma P19 cells.

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Acknowledgments

The author would like to thank Akiko Eda and Tatsunobu Fukushima for their helpful assistance. This work was supported by a research grant from the Ministry of Health, Labor, and Welfare of Japan and by a Grant-in-Aid from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. National Institute of Neuroscience, NCNP, Kodaira, Tokyo, Japan

    Hirohiko Hohjoh

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  1. Hirohiko Hohjoh
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Correspondence to Hirohiko Hohjoh .

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Editors and Affiliations

  1. Keck School of Medicine, Dept. of Cell and Neurobiology, University of Southern California, San Pablo St. 1333, Los Angeles, 90033, California, USA

    Shao-Yao Ying

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Hohjoh, H. (2013). MicroRNA Expression During Neuronal Differentiation of Human Teratocarcinoma NTera2D1 and Mouse Embryonic Carcinoma P19 Cells. In: Ying, SY. (eds) MicroRNA Protocols. Methods in Molecular Biology, vol 936. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-083-0_20

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  • DOI: https://doi.org/10.1007/978-1-62703-083-0_20

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-082-3

  • Online ISBN: 978-1-62703-083-0

  • eBook Packages: Springer Protocols

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