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Retinal Degeneration pp 113–125Cite as

Generation of Transgenic X. laevis Models of Retinal Degeneration

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 935))

Abstract

Transgenic models are invaluable tools for researching retinal degenerative disease mechanisms. However, they are time-consuming and expensive to generate and maintain. We have developed an alternative to transgenic rodent models of retinal degeneration using transgenic Xenopus laevis. We have optimized this system to allow rapid analysis of transgene effects in primary transgenic animals, thereby providing an alternative to establishing transgenic lines, and simultaneously allowing rigorous comparisons between the effects of different transgenes.

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Acknowledgments

This research was funded by the Canadian Institutes for Health Research, and the Foundation Fighting Blindness (Canada).

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Authors and Affiliations

  1. Department of Ophthalmology & Visual Sciences, University of British Columbia, Vancouver, BC, Canada

    Beatrice M. Tam, Christine C.-L. Lai, Zusheng Zong & Orson L. Moritz

Authors
  1. Beatrice M. Tam
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  2. Christine C.-L. Lai
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  3. Zusheng Zong
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  4. Orson L. Moritz
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Corresponding author

Correspondence to Orson L. Moritz .

Editor information

Editors and Affiliations

  1. , Institute of Human Genetics, University of Regensburg, Franz-Josef-Strauss-Allee 11, Regensburg, 93053, Germany

    Bernhard H.F. Weber

  2. INSTITUTE OF HUMAN GENETICS, INSTITUTE OF HUMAN GENETICS, FRANZ-JOSEF-STRAUSS-ALLEE 11, 93053 REGENSBURG, Germany

    THOMAS LANGMANN

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Tam, B.M., Lai, C.CL., Zong, Z., Moritz, O.L. (2012). Generation of Transgenic X. laevis Models of Retinal Degeneration. In: Weber, B., LANGMANN, T. (eds) Retinal Degeneration. Methods in Molecular Biology, vol 935. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-080-9_8

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  • DOI: https://doi.org/10.1007/978-1-62703-080-9_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-079-3

  • Online ISBN: 978-1-62703-080-9

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