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Functional Phenotyping of Mouse Models with ERG

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Retinal Degeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 935))

Abstract

In many situations it is important to be able to assess the degree of retinal function, e.g., for the characterization of mouse models with unknown retinal involvement, when studying degenerative processes, for the analysis of visual signal processing, and during the follow-up of therapeutic interventions. Full-field electroretinography (ERG), yielding a sum response of event-related transient electrical activity of the entire retina to light stimulation, is widely applied in human as well as experimental functional diagnostics. ERG examinations normally include initial dark-adapted (scotopic) measurements that enable rod-driven activity to be studied, followed by light-adapted (photopic) recordings to obtain information about cone system contributions. The results allow the correlation of acute or long-term disease-related changes or their alleviation by therapy with morphological data, in order to obtain a comprehensive understanding of the underlying processes and mechanisms.

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Acknowledgements

We thank Anne Kurtenbach for critical reading of the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft (DFG, grants KFO134-Se837/5-2 and Se837/6-1) to M.W.S., and the Kerstan Foundation to N.T.

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Authors and Affiliations

  1. Division of Ocular Neurodegeneration, Centre for Ophthalmology, Institute for Ophthalmic Research, University of Tuebingen, Tuebingen, Germany

    Naoyuki Tanimoto, Vithiyanjali Sothilingam & Mathias W. Seeliger

Authors
  1. Naoyuki Tanimoto
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  2. Vithiyanjali Sothilingam
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  3. Mathias W. Seeliger
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Corresponding author

Correspondence to Naoyuki Tanimoto .

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Editors and Affiliations

  1. , Institute of Human Genetics, University of Regensburg, Franz-Josef-Strauss-Allee 11, Regensburg, 93053, Germany

    Bernhard H.F. Weber

  2. INSTITUTE OF HUMAN GENETICS, INSTITUTE OF HUMAN GENETICS, FRANZ-JOSEF-STRAUSS-ALLEE 11, 93053 REGENSBURG, Germany

    THOMAS LANGMANN

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Tanimoto, N., Sothilingam, V., Seeliger, M.W. (2012). Functional Phenotyping of Mouse Models with ERG. In: Weber, B., LANGMANN, T. (eds) Retinal Degeneration. Methods in Molecular Biology, vol 935. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-080-9_4

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  • DOI: https://doi.org/10.1007/978-1-62703-080-9_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-079-3

  • Online ISBN: 978-1-62703-080-9

  • eBook Packages: Springer Protocols

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