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High-Throughput RNA In Situ Hybridization in Mouse Retina

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Retinal Degeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 935))

Abstract

The introduction of large-scale gene expression profiling studies has greatly increased the need to rapidly obtain high-quality cellular expression patterns of genes found to exhibit differential expression. The use of large-scale nonradioactive RNA in situ hybridization makes this possible, and greatly increases the general usefulness of this data. Here, we describe protocols for parallel analysis of up to 50 different gene-specific probes in mouse retinal sections.

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Correspondence to Seth Blackshaw .

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© 2012 Springer Science+Business Media, LLC

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Blackshaw, S. (2012). High-Throughput RNA In Situ Hybridization in Mouse Retina. In: Weber, B., LANGMANN, T. (eds) Retinal Degeneration. Methods in Molecular Biology, vol 935. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-080-9_15

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  • DOI: https://doi.org/10.1007/978-1-62703-080-9_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-079-3

  • Online ISBN: 978-1-62703-080-9

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