Abstract
Terminal dUTP nick end labeling (TUNEL) is an invaluable technique used in the study of late-stage apoptosis. The technique is based upon detection of fragmented DNA, a well-recognized characteristic of apoptosis, usually with fluorescent markers. Here, we describe the TUNEL technique (1) employing two different detection techniques, fluorescence microscopy and fluorescence-activated cell sorting (2) which can be applied to the analysis of apoptosis in retinal tissues or retinal cell cultures, respectively.
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Doonan, F., Cotter, T.G. (2012). Detection of DNA Fragmentation in Retinal Apoptosis by TUNEL. In: Weber, B., LANGMANN, T. (eds) Retinal Degeneration. Methods in Molecular Biology, vol 935. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-080-9_14
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DOI: https://doi.org/10.1007/978-1-62703-080-9_14
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-080-9
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