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Analysis of Optokinetic Response in Zebrafish by Computer-Based Eye Tracking

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Retinal Degeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 935))

Abstract

Large-field movements in the visual surround trigger spontaneous, compensatory eye movements known as optokinetic response (OKR) in all vertebrates. In zebrafish (Danio rerio) the OKR is well developed at 5 days post fertilization and can be used in the laboratory for screening of visual performance following genetic manipulations or pharmaceutical treatments. Several setups for measurement of the zebrafish OKR have been described. All of them are based on the presentation of moving gratings to the larva or to the adult fish. However, they differ in the way of presenting gratings and in the method of analysis. Here, we describe a detailed protocol for our newest software that enables computer-generation of the moving stripes and automatic tracking of eye movement. This protocol makes it possible to quantitatively measure OKR in both larvae and adult fishes in a fast and reliable way.

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Author information

Authors and Affiliations

  1. Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland

    Sabina P. Huber-Reggi, Kaspar P. Mueller & Stephan C. F. Neuhauss

Authors
  1. Sabina P. Huber-Reggi
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  2. Kaspar P. Mueller
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  3. Stephan C. F. Neuhauss
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Corresponding author

Correspondence to Stephan C. F. Neuhauss .

Editor information

Editors and Affiliations

  1. , Institute of Human Genetics, University of Regensburg, Franz-Josef-Strauss-Allee 11, Regensburg, 93053, Germany

    Bernhard H.F. Weber

  2. INSTITUTE OF HUMAN GENETICS, INSTITUTE OF HUMAN GENETICS, FRANZ-JOSEF-STRAUSS-ALLEE 11, 93053 REGENSBURG, Germany

    THOMAS LANGMANN

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Huber-Reggi, S.P., Mueller, K.P., Neuhauss, S.C.F. (2012). Analysis of Optokinetic Response in Zebrafish by Computer-Based Eye Tracking. In: Weber, B., LANGMANN, T. (eds) Retinal Degeneration. Methods in Molecular Biology, vol 935. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-080-9_10

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  • DOI: https://doi.org/10.1007/978-1-62703-080-9_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-079-3

  • Online ISBN: 978-1-62703-080-9

  • eBook Packages: Springer Protocols

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