Abstract
The physiological function of all cells is uniquely regulated by changes in cytosolic Ca2+ levels. Although several mechanisms increase cytosolic Ca2+ levels, Ca2+ influx across the plasma membrane upon the release of Ca2+ from the internal stores is one of the major mechanisms in most nonexcitable cells and in some excitable cells. Such Ca2+ channels, which are activated by intracellular Ca2+ store depletion are referred to as store-operated Ca2+ entry (SOCE) channels and have been shown to be essential for many biological functions including fluid and enzyme secretion, immune regulation, hypertension, pulmonary function, neurosecretion, synaptic plasticity, and vascular diseases. Canonical transient receptor potential (TRPCs) have been proposed as components of the store-operated Ca2+ channel (SOCC) which mediates SOCE. TRPC channels are nonselective cation channels, present in a signaling complex where they interact with key proteins critical for their regulation. In this regard, there is increasing recognition that Ca2+ entry via SOCE channels plays critical roles in the lung, particularly for vascular and airway function. Indeed, regulation/targeting of TRPC channels appear to be important in normal vascular and airway physiology as well as pathophysiology of lung diseases. In this chapter, we briefly summarize the current state of knowledge regarding expression, regulation, and function of TRPC channels in the vasculature and the respiratory system.
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References
Bootman MD, Berridge MJ, Roderick HL (2002) Calcium signalling: more messengers, more channels, more complexity. Curr Biol 12:R563–565
Berridge MJ, Lipp P, Bootman MD (2000) The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol 1:11–21
Berridge MJ, Bootman MD, Roderick HL (2003) Calcium signalling: dynamics, homeostasis and remodelling. Nat Rev Mol Cell Biol 4:517–529
Parekh AB, Putney JW Jr (2005) Store-operated calcium channels. Physiol Rev 85:757–810
Putney JW Jr (2004) Store-operated calcium channels: how do we measure them, and why do we care? Sci STKE 2004:pe37
Putney JW Jr (1993) Excitement about calcium signaling in inexcitable cells. Science 262:676–678
Putney JW Jr (1990) Capacitative calcium entry revisited. Cell Calcium 11:611–624
Putney JW (2011) Origins of the concept of store-operated calcium entry. Front Biosci (Schol Ed) 3:980–984
Ambudkar IS, Brazer SC, Liu X, Lockwich T, Singh B (2004) Plasma membrane localization of TRPC channels: role of caveolar lipid rafts. Novartis Found Symp 258:63–70, discussion 70–64, 98–102, 263–106
Birnbaumer L et al (2000) Mechanism of capacitative Ca2+ entry (CCE): interaction between IP3 receptor and TRP links the internal calcium storage compartment to plasma membrane CCE channels. Recent Prog Horm Res 55:127–161, discussion 161–122
Montell C (2005) The TRP superfamily of cation channels. Sci STKE 2005:re3
Montell C (1999) Visual transduction in Drosophila. Annu Rev Cell Dev Biol 15:231–268
Pani B, Singh BB (2009) Lipid rafts/caveolae as microdomains of calcium signaling. Cell Calcium 45:625–633
Ambudkar IS, Ong HL (2007) Organization and function of TRPC channelosomes. Pflugers Arch 455:187–200
Singh BB, Liu X, Tang J, Zhu MX, Ambudkar IS (2002) Calmodulin regulates Ca2+-dependent feedback inhibition of store-operated Ca2+ influx by interaction with a site in the C terminus of TrpC1. Mol Cell 9:739–750
Zhu MX (2005) Multiple roles of calmodulin and other Ca2+-binding proteins in the functional regulation of TRP channels. Pflugers Arch 451:105–115
Niemeyer BA, Bergs C, Wissenbach U, Flockerzi V, Trost C (2001) Competitive regulation of CaT-like-mediated Ca2+ entry by protein kinase C and calmodulin. Proc Natl Acad Sci USA 98:3600–3605
DeMaria CD, Soong TW, Alseikhan BA, Alvania RS, Yue DT (2001) Calmodulin bifurcates the local Ca2+ signal that modulates P/Q-type Ca2+ channels. Nature 411:484–489
Moreau B, Straube S, Fisher RJ, Putney JW Jr, Parekh AB (2005) Ca2+  -calmodulin-dependent facilitation and Ca2+ inactivation of Ca2+ release-activated Ca2+ channels. J Biol Chem 280:8776–8783
Zhu X et al (1996) trp, a novel mammalian gene family essential for agonist-activated capacitative Ca2+ entry. Cell 85:661–671
Minke B, Cook B (2002) TRP channel proteins and signal transduction. Physiol Rev 82:429–472
Venkatachalam K, Zheng F, Gill DL (2003) Regulation of canonical transient receptor potential (TRPC) channel function by diacylglycerol and protein kinase C. J Biol Chem 278:29031–29040
Kiselyov K, Mignery GA, Zhu MX, Muallem S (1999) The N-terminal domain of the IP3 receptor gates store-operated hTrp3 channels. Mol Cell 4:423–429
Kiselyov KI et al (2000) Gating of store-operated channels by conformational coupling to ryanodine receptors. Mol Cell 6:421–431
Lockwich T, Singh BB, Liu X, Ambudkar IS (2001) Stabilization of cortical actin induces internalization of transient receptor potential 3 (Trp3)-associated caveolar Ca2+ signaling complex and loss of Ca2+ influx without disruption of Trp3-inositol trisphosphate receptor association. J Biol Chem 276:42401–42408
Ahmmed GU et al (2004) Protein kinase Calpha phosphorylates the TRPC1 channel and regulates store-operated Ca2+ entry in endothelial cells. J Biol Chem 279:20941–20949
Kwan HY, Huang Y, Yao X (2006) Protein kinase C can inhibit TRPC3 channels indirectly via stimulating protein kinase G. J Cell Physiol 207:315–321
Vazquez G, Wedel BJ, Kawasaki BT, Bird GS, Putney JW Jr (2004) Obligatory role of Src kinase in the signaling mechanism for TRPC3 cation channels. J Biol Chem 279:40521–40528
Hisatsune C et al (2004) Regulation of TRPC6 channel activity by tyrosine phosphorylation. J Biol Chem 279:18887–18894
Shi J et al (2004) Multiple regulation by calcium of murine homologues of transient receptor potential proteins TRPC6 and TRPC7 expressed in HEK293 cells. J Physiol 561:415–432
Boulay G et al (1999) Modulation of Ca2+ entry by polypeptides of the inositol 1,4, 5-trisphosphate receptor (IP3R) that bind transient receptor potential (TRP): evidence for roles of TRP and IP3R in store depletion-activated Ca2+ entry. Proc Natl Acad Sci USA 96:14955–14960
Vaca L, Sampieri A (2002) Calmodulin modulates the delay period between release of calcium from internal stores and activation of calcium influx via endogenous TRP1 channels. J Biol Chem 277:42178–42187
Liou J et al (2005) STIM is a Ca2+ sensor essential for Ca2+  -store-depletion-triggered Ca2+ influx. Curr Biol 15:1235–1241
Roos J et al (2005) STIM1, an essential and conserved component of store-operated Ca2+ channel function. J Cell Biol 169:435–445
Zeng W et al (2008) STIM1 gates TRPC channels, but not Orai1, by electrostatic interaction. Mol Cell 32:439–448
Worley PF et al (2007) TRPC channels as STIM1-regulated store-operated channels. Cell Calcium 42:205–211
Manji SS et al (2000) STIM1: a novel phosphoprotein located at the cell surface. Biochim Biophys Acta 1481:147–155
Williams RT et al (2001) Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins. Biochem J 357:673–685
Zhang SL et al (2005) STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature 437:902–905
Brandman O, Liou J, Park WS, Meyer T (2007) STIM2 is a feedback regulator that stabilizes basal cytosolic and endoplasmic reticulum Ca2+ levels. Cell 131:1327–1339
Smyth JT et al (2009) Phosphorylation of STIM1 underlies suppression of store-operated calcium entry during mitosis. Nat Cell Biol 11:1465–1472
Yu F, Sun L, Machaca K (2009) Orai1 internalization and STIM1 clustering inhibition modulate SOCE inactivation during meiosis. Proc Natl Acad Sci USA 106:17401–17406
Liou J, Fivaz M, Inoue T, Meyer T (2007) Live-cell imaging reveals sequential oligomerization and local plasma membrane targeting of stromal interaction molecule 1 after Ca2+ store depletion. Proc Natl Acad Sci USA 104:9301–9306
Luik RM, Wang B, Prakriya M, Wu MM, Lewis RS (2008) Oligomerization of STIM1 couples ER calcium depletion to CRAC channel activation. Nature 454:538–542
Luik RM, Wu MM, Buchanan J, Lewis RS (2006) The elementary unit of store-operated Ca2+ entry: local activation of CRAC channels by STIM1 at ER-plasma membrane junctions. J Cell Biol 174:815–825
Muik M et al (2008) Dynamic coupling of the putative coiled-coil domain of ORAI1 with STIM1 mediates ORAI1 channel activation. J Biol Chem 283:8014–8022
Wu MM, Buchanan J, Luik RM, Lewis RS (2006) Ca2+ store depletion causes STIM1 to accumulate in ER regions closely associated with the plasma membrane. J Cell Biol 174:803–813
Xu P et al (2006) Aggregation of STIM1 underneath the plasma membrane induces clustering of Orai1. Biochem Biophys Res Commun 350:969–976
Pani B et al (2008) Lipid rafts determine clustering of STIM1 in endoplasmic reticulum-plasma membrane junctions and regulation of store-operated Ca2+ entry (SOCE). J Biol Chem 283:17333–17340
Kim MS et al (2009) Native Store-operated Ca2+ influx requires the channel function of orai1 and TRPC1. J Biol Chem 284:9733–9741
Yuan JP et al (2009) SOAR and the polybasic STIM1 domains gate and regulate Orai channels. Nat Cell Biol 11:337–343
Cheng KT, Ong HL, Liu X, Ambudkar IS (2011) Contribution of TRPC1 and Orai1 to Ca2+ entry activated by store depletion. Adv Exp Med Biol 704:435–449
Salido GM, Jardin I, Rosado JA (2011) The TRPC ion channels: association with orai1 and STIM1 proteins and participation in capacitative and non-capacitative calcium entry. Adv Exp Med Biol 704:413–433
Lee KP et al (2010) An endoplasmic reticulum/plasma membrane junction: STIM1/Orai1/TRPCs. FEBS Lett 584:2022–2027
Lee KP, Yuan JP, So I, Worley PF, Muallem S (2010) STIM1-dependent and STIM1-independent function of TRPC channels tunes their store-operated mode. J Biol Chem 285:38666–38673
Prakash YS et al (2007) Caveolins and intracellular calcium regulation in human airway smooth muscle. Am J Physiol Lung Cell Mol Physiol 293:L1118–1126
Isshiki M et al (2002) Sites of Ca2+ wave initiation move with caveolae to the trailing edge of migrating cells. J Cell Sci 115:475–484
Guo B, Kato RM, Garcia-Lloret M, Wahl MI, Rawlings DJ (2000) Engagement of the human pre-B cell receptor generates a lipid raft-dependent calcium signaling complex. Immunity 13:243–253
Tiruppathi C, Ahmmed GU, Vogel SM, Malik AB (2006) Ca2+ signaling, TRP channels, and endothelial permeability. Microcirculation 13:693–708
Lin RC, Scheller RH (2000) Mechanisms of synaptic vesicle exocytosis. Annu Rev Cell Dev Biol 16:19–49
Singh BB et al (2004) VAMP2-dependent exocytosis regulates plasma membrane insertion of TRPC3 channels and contributes to agonist-stimulated Ca2+ influx. Mol Cell 15:635–646
Bezzerides VJ, Ramsey IS, Kotecha S, Greka A, Clapham DE (2004) Rapid vesicular translocation and insertion of TRP channels. Nat Cell Biol 6:709–720
Cayouette S, Lussier MP, Mathieu EL, Bousquet SM, Boulay G (2004) Exocytotic insertion of TRPC6 channel into the plasma membrane upon Gq protein-coupled receptor activation. J Biol Chem 279:7241–7246
Bollimuntha S, Cornatzer E, Singh BB (2005) Plasma membrane localization and function of TRPC1 is dependent on its interaction with beta-tubulin in retinal epithelium cells. Vis Neurosci 22:163–170
Smyth JT, DeHaven WI, Bird GS, Putney JW Jr (2007) Role of the microtubule cytoskeleton in the function of the store-operated Ca2+ channel activator STIM1. J Cell Sci 120:3762–3771
Earley S, Brayden JE (2010) Transient receptor potential channels and vascular function. Clin Sci 119:19–36
Moore T et al (1998) Regulation of pulmonary endothelial cell shape by Trp-mediated calcium entry. Chest 114:36S–38S
Moore TM et al (1998) Store-operated calcium entry promotes shape change in pulmonary endothelial cells expressing Trp1. Am J Physiol 275:L574–582
Brough GH et al (2001) Contribution of endogenously expressed Trp1 to a Ca2+  -selective, store-operated Ca2+ entry pathway. FASEB J 15:1727–1738, official publication of the Federation of American Societies for Experimental Biology
Groschner K et al (1998) Trp proteins form store-operated cation channels in human vascular endothelial cells. FEBS Lett 437:101–106
Freichel M et al (2001) Lack of an endothelial store-operated Ca2+ current impairs agonist-dependent vasorelaxation in TRP4−/− mice. Nat Cell Biol 3:121–127
Paria BC et al (2003) Tumor necrosis factor-alpha induces nuclear factor-kappaB-dependent TRPC1 expression in endothelial cells. J Biol Chem 278:37195–37203
Di A, Malik AB (2010) TRP channels and the control of vascular function. Curr Opin Pharmacol 10:127–132
Cheng KT, Ong HL, Liu X, Ambudkar IS (2011) Contribution of TRPC1 and Orai1 to Ca2+ entry activated by store depletion. Adv Exp Med Biol 704:435–449
Hogan PG, Lewis RS, Rao A (2010) Molecular basis of calcium signaling in lymphocytes: STIM and ORAI. Annu Rev Immunol 28:491–533
Lee KP et al (2010) An endoplasmic reticulum/plasma membrane junction: STIM1/Orai1/TRPCs. FEBS Lett 584:2022–2027
Putney JW (2010) Pharmacology of store-operated calcium channels. Mol Interv 10:209–218
Schindl R et al (2009) Recent progress on STIM1 domains controlling Orai activation. Cell Calcium 46:227–232
Yuan JP et al (2009) TRPC channels as STIM1-regulated SOCs. Channels 3:221–225
Abdullaev IF et al (2008) Stim1 and Orai1 mediate CRAC currents and store-operated calcium entry important for endothelial cell proliferation. Circ Res 103:1289–1299
Patel HH et al (2007) Increased smooth muscle cell expression of caveolin-1 and caveolae contribute to the pathophysiology of idiopathic pulmonary arterial hypertension. FASEB J 21:2970–2979, Official publication of the Federation of American Societies for Experimental Biology
Remillard CV, Yuan JX (2006) Transient receptor potential channels and caveolin-1: good friends in tight spaces. Mol Pharmacol 70:1151–1154
Kwiatek AM et al (2006) Caveolin-1 regulates store-operated Ca2+ influx by binding of its scaffolding domain to transient receptor potential channel-1 in endothelial cells. Mol Pharmacol 70:1174–1183
Sathish V et al (2011) Caveolin-1 in cytokine-induced enhancement of intracellular Ca2+ in human airway smooth muscle. Am J Physiol Lung Cell Mol Physiol 301:607–614
Mehta D et al (2003) RhoA interaction with inositol 1,4,5-trisphosphate receptor and transient receptor potential channel-1 regulates Ca2+ entry. Role in signaling increased endothelial permeability. J Biol Chem 278:33492–33500
Jho D et al (2005) Angiopoietin-1 opposes VEGF-induced increase in endothelial permeability by inhibiting TRPC1-dependent Ca2 influx. Circ Res 96:1282–1290
Liu CL, Huang Y, Ngai CY, Leung YK, Yao XQ (2006) TRPC3 is involved in flow- and bradykinin-induced vasodilation in rat small mesenteric arteries. Acta Pharmacol Sin 27:981–990
Peppiatt-Wildman CM, Albert AP, Saleh SN, Large WA (2007) Endothelin-1 activates a Ca2+  -permeable cation channel with TRPC3 and TRPC7 properties in rabbit coronary artery myocytes. J Physiol 580:755–764
Smedlund K, Vazquez G (2008) Involvement of native TRPC3 proteins in ATP-dependent expression of VCAM-1 and monocyte adherence in coronary artery endothelial cells. Arterioscler Thromb Vasc Biol 28:2049–2055
Thilo F, Loddenkemper C, Berg E, Zidek W, Tepel M (2009) Increased TRPC3 expression in vascular endothelium of patients with malignant hypertension. Mod Pathol 22:426–430, An official journal of the United States and Canadian Academy of Pathology, Inc
Xi Q et al (2008) IP3 constricts cerebral arteries via IP3 receptor-mediated TRPC3 channel activation and independently of sarcoplasmic reticulum Ca2+ release. Circ Res 102:1118–1126
Balzer M, Lintschinger B, Groschner K (1999) Evidence for a role of Trp proteins in the oxidative stress-induced membrane conductances of porcine aortic endothelial cells. Cardiovasc Res 42:543–549
Hill AJ et al (2006) A TRPC-like non-selective cation current activated by alpha 1-adrenoceptors in rat mesenteric artery smooth muscle cells. Cell Calcium 40:29–40
Eder P et al (2007) Phospholipase C-dependent control of cardiac calcium homeostasis involves a TRPC3-NCX1 signaling complex. Cardiovasc Res 73:111–119
Kwan HY, Huang Y, Yao X (2007) TRP channels in endothelial function and dysfunction. Biochim Biophys Acta 1772:907–914
Tiruppathi C et al (2002) Impairment of store-operated Ca2+ entry in TRPC4(-/-) mice interferes with increase in lung microvascular permeability. Circ Res 91:70–76
Tiruppathi C, Minshall RD, Paria BC, Vogel SM, Malik AB (2002) Role of Ca2+ signaling in the regulation of endothelial permeability. Vascul Pharmacol 39:173–185
Chaudhuri P et al (2008) Elucidation of a TRPC6-TRPC5 channel cascade that restricts endothelial cell movement. Mol Biol Cell 19:3203–3211
Yoshida T et al (2006) Nitric oxide activates TRP channels by cysteine S-nitrosylation. Nat Chem Biol 2:596–607
Wong CO, Sukumar P, Beech DJ, Yao X (2010) Nitric oxide lacks direct effect on TRPC5 channels but suppresses endogenous TRPC5-containing channels in endothelial cells. Pflugers Arch 460:121–130
Ge R et al (2009) Critical role of TRPC6 channels in VEGF-mediated angiogenesis. Cancer Lett 283:43–51
Singh I et al (2007) Galphaq-TRPC6-mediated Ca2+ entry induces RhoA activation and resultant endothelial cell shape change in response to thrombin. J Biol Chem 282:7833–7843
Leung PC et al (2006) Mechanism of non-capacitative Ca2+ influx in response to bradykinin in vascular endothelial cells. J Vasc Res 43:367–376
Cheng HW, James AF, Foster RR, Hancox JC, Bates DO (2006) VEGF activates receptor-operated cation channels in human microvascular endothelial cells. Arterioscler Thromb Vasc Biol 26:1768–1776
Yao X, Garland CJ (2005) Recent developments in vascular endothelial cell transient receptor potential channels. Circ Res 97:853–863
Albert AP, Saleh SN, Large WA (2009) Identification of canonical transient receptor potential (TRPC) channel proteins in native vascular smooth muscle cells. Curr Med Chem 16:1158–1165
Beech DJ (2005) Emerging functions of 10 types of TRP cationic channel in vascular smooth muscle. Clin Exp Pharmacol Physiol 32:597–603
Brayden JE, Earley S, Nelson MT, Reading S (2008) Transient receptor potential (TRP) channels, vascular tone and autoregulation of cerebral blood flow. Clin Exp Pharmacol Physiol 35:1116–1120
Gonzalez-Cobos JC, Trebak M (2010) TRPC channels in smooth muscle cells. Front Biosci 15:1023–1039, a journal and virtual library
House SJ, Potier M, Bisaillon J, Singer HA, Trebak M (2008) The non-excitable smooth muscle: calcium signaling and phenotypic switching during vascular disease. Pflugers Arch 456:769–785
Landsberg JW, Yuan JX (2004) Calcium and TRP channels in pulmonary vascular smooth muscle cell proliferation. News Physiol Sci 19:44–50, An international journal of physiology produced jointly by the International Union of Physiological Sciences and the American Physiological Society
Remillard CV, Yuan JX (2006) TRP channels, CCE, and the pulmonary vascular smooth muscle. Microcirculation 13:671–692
Wamhoff BR, Bowles DK, Owens GK (2006) Excitation-transcription coupling in arterial smooth muscle. Circ Res 98:868–878
Guibert C, Ducret T, Savineau JP (2011) Expression and physiological roles of TRP channels in smooth muscle cells. Adv Exp Med Biol 704:687–706
Saleh SN, Albert AP, Peppiatt CM, Large WA (2006) Angiotensin II activates two cation conductances with distinct TRPC1 and TRPC6 channel properties in rabbit mesenteric artery myocytes. J Physiol 577:479–495
Tai K et al (2008) Agonist-evoked calcium entry in vascular smooth muscle cells requires IP3 receptor-mediated activation of TRPC1. Eur J Pharmacol 583:135–147
Dietrich A, Kalwa H, Gudermann T (2010) TRPC channels in vascular cell function. Thromb Haemost 103:262–270
Tang C, To WK, Meng F, Wang Y, Gu Y (2010) A role for receptor-operated Ca2+ entry in human pulmonary artery smooth muscle cells in response to hypoxia. Physiol Res 59:909–918
Kunichika N et al (2004) Overexpression of TRPC1 enhances pulmonary vasoconstriction induced by capacitative Ca2+ entry. Am J Physiol Lung Cell Mol Physiol 287:L962–969
Bergdahl A et al (2005) Plasticity of TRPC expression in arterial smooth muscle: correlation with store-operated Ca2+ entry. Am J Physiol Cell Physiol 288:C872–880
Dietrich A et al (2007) Pressure-induced and store-operated cation influx in vascular smooth muscle cells is independent of TRPC1. Pflugers Arch 455:465–477
Takahashi S et al (2008) Nitric oxide-cGMP-protein kinase G pathway negatively regulates vascular transient receptor potential channel TRPC6. J Physiol 586:4209–4223
Sweeney M et al (2002) Inhibition of endogenous TRP1 decreases capacitative Ca2+ entry and attenuates pulmonary artery smooth muscle cell proliferation. Am J Physiol Lung Cell Mol Physiol 283:L144–155
Li J et al (2008) Interactions, functions, and independence of plasma membrane STIM1 and TRPC1 in vascular smooth muscle cells. Circ Res 103:e97–104
Gudermann T, Hofmann T, Mederos y Schnitzler M, Dietrich A (2004) Activation, subunit composition and physiological relevance of DAG-sensitive TRPC proteins. Novartis Found Symp 258:103–118, discussion 118-122, 155-109, 263-106
Hofmann T et al (1999) Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol. Nature 397:259–263
Chen J, Crossland RF, Noorani MM, Marrelli SP (2009) Inhibition of TRPC1/TRPC3 by PKG contributes to NO-mediated vasorelaxation. Am J Physiol Heart Circ Physiol 297:H417–424
Liu D et al (2009) Increased transient receptor potential canonical type 3 channels in vasculature from hypertensive rats. Hypertension 53:70–76
Xie A et al (2007) Novel mechanism of endothelin-1-induced vasospasm after subarachnoid hemorrhage. J Cereb Blood Flow Metab 27:1692–1701, Official journal of the International Society of Cerebral Blood Flow and Metabolism
Lindsey SH, Tribe RM, Songu-Mize E (2008) Cyclic stretch decreases TRPC4 protein and capacitative calcium entry in rat vascular smooth muscle cells. Life Sci 83:29–34
Xu SZ, Boulay G, Flemming R, Beech DJ (2006) E3-targeted anti-TRPC5 antibody inhibits store-operated calcium entry in freshly isolated pial arterioles. Am J Physiol Heart Circ Physiol 291:H2653–2659
Xu SZ et al (2006) A sphingosine-1-phosphate-activated calcium channel controlling vascular smooth muscle cell motility. Circ Res 98:1381–1389
Flemming PK et al (2006) Sensing of lysophospholipids by TRPC5 calcium channel. J Biol Chem 281:4977–4982
Shi J, Ju M, Saleh SN, Albert AP, Large WA (2010) TRPC6 channels stimulated by angiotensin II are inhibited by TRPC1/C5 channel activity through a Ca2+  - and PKC-dependent mechanism in native vascular myocytes. J Physiol 588:3671–3682
Zulian A et al (2010) Upregulation of Na+/Ca2+ exchanger and TRPC6 contributes to abnormal Ca2+ homeostasis in arterial smooth muscle cells from Milan hypertensive rats. Am J Physiol Heart Circ Physiol 299:H624–633
Graham S et al (2010) Canonical transient receptor potential 6 (TRPC6), a redox-regulated cation channel. J Biol Chem 285:23466–23476
Erac Y, Selli C, Kosova B, Akcali KC, Tosun M (2010) Expression levels of TRPC1 and TRPC6 ion channels are reciprocally altered in aging rat aorta: implications for age-related vasospastic disorders. Age 32:223–230
Ju M, Shi J, Saleh SN, Albert AP, Large WA (2010) Ins(1,4,5)P3 interacts with PIP2 to regulate activation of TRPC6/C7 channels by diacylglycerol in native vascular myocytes. J Physiol 588:1419–1433
Albert AP, Saleh SN, Large WA (2008) Inhibition of native TRPC6 channel activity by phosphatidylinositol 4,5-bisphosphate in mesenteric artery myocytes. J Physiol 586:3087–3095
Bae YM et al (2007) Enhancement of receptor-operated cation current and TRPC6 expression in arterial smooth muscle cells of deoxycorticosterone acetate-salt hypertensive rats. J Hypertens 25:809–817
Jung S, Strotmann R, Schultz G, Plant TD (2002) TRPC6 is a candidate channel involved in receptor-stimulated cation currents in A7r5 smooth muscle cells. Am J Physiol Cell Physiol 282:C347–359
Inoue R et al (2001) The transient receptor potential protein homologue TRP6 is the essential component of vascular alpha(1)-adrenoceptor-activated Ca2+-permeable cation channel. Circ Res 88:325–332
Soboloff J et al (2005) Role of endogenous TRPC6 channels in Ca2+ signal generation in A7r5 smooth muscle cells. J Biol Chem 280:39786–39794
Poburko D, Fameli N, Kuo KH, van Breemen C (2008) Ca2+ signaling in smooth muscle: TRPC6. NCX and LNats in nanodomains. Channels (Austin) 2:10–12
Fellner SK, Arendshorst WJ (2008) Angiotensin II-stimulated Ca2+ entry mechanisms in afferent arterioles: role of transient receptor potential canonical channels and reverse Na+/Ca2+ exchange. Am J Physiol Renal Physiol 294:F212–219
Welsh DG, Morielli AD, Nelson MT, Brayden JE (2002) Transient receptor potential channels regulate myogenic tone of resistance arteries. Circ Res 90:248–250
Yu Y et al (2004) Enhanced expression of transient receptor potential channels in idiopathic pulmonary arterial hypertension. Proc Natl Acad Sci USA 101:13861–13866
Yu Y et al (2003) PDGF stimulates pulmonary vascular smooth muscle cell proliferation by upregulating TRPC6 expression. Am J Physiol Cell Physiol 284:C316–330
Weissmann N et al (2006) Classical transient receptor potential channel 6 (TRPC6) is essential for hypoxic pulmonary vasoconstriction and alveolar gas exchange. Proc Natl Acad Sci USA 103:19093–19098
Walker RL, Hume JR, Horowitz B (2001) Differential expression and alternative splicing of TRP channel genes in smooth muscles. Am J Physiol Cell Physiol 280:C1184–1192
George SJ, Dwivedi A (2004) MMPs, cadherins, and cell proliferation. Trends Cardiovasc Med 14:100–105
Hedin U, Roy J, Tran PK (2004) Control of smooth muscle cell proliferation in vascular disease. Curr Opin Lipidol 15:559–565
Rivard A, Andres V (2000) Vascular smooth muscle cell proliferation in the pathogenesis of atherosclerotic cardiovascular diseases. Histol Histopathol 15:557–571
Sanz-Gonzalez SM et al (2000) Control of vascular smooth muscle cell growth by cyclin-dependent kinase inhibitory proteins and its implication in cardiovascular disease. Front Biosci 5:D619–628, A journal and virtual library
Schachter M (1997) Vascular smooth muscle cell migration, atherosclerosis, and calcium channel blockers. Int J Cardiol 62(Suppl 2):S85–90
Sriram V, Patterson C (2001) Cell cycle in vasculoproliferative diseases: potential interventions and routes of delivery. Circulation 103:2414–2419
Afroze T, Husain M (2001) Cell cycle dependent regulation of intracellular calcium concentration in vascular smooth muscle cells: a potential target for drug therapy. Curr Drug Targets Cardiovasc Haematol Disord 1:23–40
Mason RP, Marche P, Hintze TH (2003) Novel vascular biology of third-generation L-type calcium channel antagonists: ancillary actions of amlodipine. Arterioscler Thromb Vasc Biol 23:2155–2163
Schachter M (1997) Calcium antagonists and atherosclerosis. Int J Cardiol 62(Suppl 2):S9–15
Banner KH, Igney F, Poll C (2011) TRP channels: emerging targets for respiratory disease. Pharmacol Ther 130:371–384
Li S, Westwick J, Cox B, Poll CT (2004) TRP channels as drug targets. Novartis Found Symp 258:204–213, discussion 213–221, 263–206
Li S, Westwick J, Poll C (2003) Transient receptor potential (TRP) channels as potential drug targets in respiratory disease. Cell Calcium 33:551–558
Watanabe H (2009) Pathological role of TRP channels in cardiovascular and respiratory diseases. Nihon Yakurigaku Zasshi 134:127–130, Folia pharmacologica Japonica
Corteling RL et al (2004) Expression of transient receptor potential C6 and related transient receptor potential family members in human airway smooth muscle and lung tissue. Am J Respir Cell Mol Biol 30:145–154
Antigny F et al (2011) Transient receptor potential canonical channel 6 links Ca2+ mishandling to cystic fibrosis transmembrane conductance regulator channel dysfunction in cystic fibrosis. Am J Respir Cell Mol Biol 44:83–90
Gosling M, Poll C, Li S (2005) TRP channels in airway smooth muscle as therapeutic targets. Naunyn Schmiedebergs Arch Pharmacol 371:277–284
Nassini R, Materazzi S, De Siena G, De Cesaris F, Geppetti P (2010) Transient receptor potential channels as novel drug targets in respiratory diseases. Curr Opin Investig Drugs 11:535–542
Nilius B (2007) TRP channels in disease. Biochim Biophys Acta 1772:805–812
Wang YX, Zheng YM (2011) Molecular expression and functional role of canonical transient receptor potential channels in airway smooth muscle cells. Adv Exp Med Biol 704:731–747
Ong HL, Brereton HM, Harland ML, Barritt GJ (2003) Evidence for the expression of transient receptor potential proteins in guinea pig airway smooth muscle cells. Respirology 8:23–32
White TA et al (2006) Role of transient receptor potential C3 in TNF-alpha-enhanced calcium influx in human airway myocytes. Am J Respir Cell Mol Biol 35:243–251
Ong HL et al (2002) Specific detection of the endogenous transient receptor potential (TRP)-1 protein in liver and airway smooth muscle cells using immunoprecipitation and Western-blot analysis. Biochem J 364:641–648
Godin N, Rousseau E (2007) TRPC6 silencing in primary airway smooth muscle cells inhibits protein expression without affecting OAG-induced calcium entry. Mol Cell Biochem 296:193–201
Ay B, Prakash YS, Pabelick CM, Sieck GC (2004) Store-operated Ca2+ entry in porcine airway smooth muscle. Am J Physiol Lung Cell Mol Physiol 286:L909–917
Xiao JH, Zheng YM, Liao B, Wang YX (2010) Functional role of canonical transient receptor potential 1 and canonical transient receptor potential 3 in normal and asthmatic airway smooth muscle cells. Am J Respir Cell Mol Biol 43:17–25
Rosker C et al (2004) Ca2+ signaling by TRPC3 involves Na+ entry and local coupling to the Na+/Ca2+ exchanger. J Biol Chem 279:13696–13704
Liu XS, Xu YJ (2005) Potassium channels in airway smooth muscle and airway hyperreactivity in asthma. Chin Med J (Engl) 118:574–580
Damann N, Owsianik G, Li S, Poll C, Nilius B (2009) The calcium-conducting ion channel transient receptor potential canonical 6 is involved in macrophage inflammatory protein-2-induced migration of mouse neutrophils. Acta Physiol (Oxf) 195:3–11
Finney-Hayward TK et al (2010) Expression of transient receptor potential c6 channels in human lung macrophages. Am J Respir Cell Mol Biol 43:296–304
Sel S et al (2008) Loss of classical transient receptor potential 6 channel reduces allergic airway response. Clin Exp Allergy 38:1548–1558, Journal of the British Society for Allergy and Clinical Immunology
Acknowledgments
This work was supported by NIH grants awarded to Brij B Singh (award number RO1 DE 017102-06 and 5P20RR017699), Christina M. Pabelick (R01 HL090595) and Y.S. Prakash (R01 HL088029).
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Singh, B.B., Pabelick, C.M., Prakash, Y.S. (2012). Canonical Transient Receptor Potential Channel Expression, Regulation, and Function in Vascular and Airway Diseases. In: Szallasi, A., BÃró, T. (eds) TRP Channels in Drug Discovery. Methods in Pharmacology and Toxicology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-077-9_4
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