Abstract
Peanut (Arachis hypogaea L.), also known as groundnut, is the most important species of Arachis genus, originating from Brazil and Peru. Peanut seeds contain high seed oil, proteins, amino acids, and vitamin E, and are consumed worldwide as edible nut, peanut butter, or candy, and peanut oil extracted from the seeds. The meal remaining after oil extraction is also used for animal feed. However, its narrow germplasm base, together with susceptibility to diseases, pathogens, and weeds, decreases yield and seed quality and causes great economic losses annually. Hence, the optimization of efficient in vitro propagation procedures would be highly effective for peanut propagation, as it would raise yield and improve seed quality and flavor. Earlier reports on traditional micropropagation methods, based on axillary bud proliferation which guarantees the multiplication of true-to-type plants, are still limited. This chapter describes a micropropagation protocol to improve multiple shoot formation from shoot-tip explants by using AgNO3 in combination with plant growth regulators.
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Stalker HT (1997) Peanut (Arachis hypogaea L.). Field Crops Res 53:205–217
Hammons RO (1982) Origin and early history of the peanut. In: Pattee HE, Young CT (eds) Peanut science and technology. American Peanut Research and Education Society, Yoakum
Pacheco G, Gagliardi RF, Valls JFM, Mansur E (2009) Micropropagation and in vitro conservation of wild Arachis species. Plant Cell Tissue Org Cult 99:239–249
Knauft DA, Ozias-Akins P (1995) Recent methodologies for germplasm enhancement and breeding. In: Pattee HE, Stalker HT (eds) Advances in Peanut Science. American Peanut Research and Education Society, Stillwater
Vásquez-Yanes C, Aréchiga MR (1996) Ex situ conservation of tropical rain forest seed: problems and perspectives. Interciencia 21:293–298
Wilcut JW, York AC, Grichar WJ, Wehtje GR (1995) The biology and management of weeds in peanut (Arachis hypogaea). In: Pattee HE, Stalker HT (eds) Advances in Peanut Science. Am Peanut Res Educ Soc Inc, Stillwater, OK
Akasaka Y, Daimon H, Mii M (2000) Improved plant regeneration from cultured leaf segments in peanut (Arachis hypogaea L.) by limited exposure to thidiazuron. Plant Sci 156:169–175
Ozudogru EA, Ozden-Tokatli Y, Akcin A (2005) Effect of silver nitrate on multiple shoot formation of virginia-type peanut through shoot tip culture. In Vitro Cell Dev Biol Plant 41:151–156
Kanyand M, Peterson CM, Prakash CS (1997) The differentiation of emergences into adventitious shoots in peanut, Arachis hypogaea (L.). Plant Sci 126:87–95
Ponsamuel J, Huhman DV, Cassidy BG, Post-Beittenmiller D (1998) In vitro regeneration via caulogenesis and brassin-induced shoot conversion of dormant buds from plumular explants of peanut (Arachis hypogaea L. Cv. ‘Okrun’). Plant Cell Rep 17:373–378
Banerjee P, Maity S, Maiti S, Banerjee N (2007) Influence of genotype on in vitro multiplication potential of Arachis hypogaea L. Acta Bot Croat 66(1):15–23
Kanyand M, Dessai AP, Prakash CS (1994) Thidiazuron promotes high frequency regeneration of peanut (Arachis hypogaea) plants in vitro. Plant Cell Rep 14:1–5
Zhijan L, Jarret RL, Pittman RN, Demski JW (1994) Shoot organogenesis from cultured seed explants of peanut (Arachis hypogaea L.) using thidiazuron. In Vitro Cell Dev – Biol Plant 30:187–191
Pestana MC, Lacorte C, De Freitas VG, De Oliveira DE, Mansur E (1999) In vitro regeneration of peanut (Arachis hypogaea L.) through organogenesis: effect of culture temperature and silver nitrate. In Vitro Cell Dev Biol Plant 35:214–216
Victor JMR, Murch SJ, KrishnaRaj S, Saxena PK (1999) Somatic embryogenesis and organogenesis in peanut: the role of thidiazuron and N6-benzylaminopurine in the induction of plant morphogenesis. Plant Growth Regul 28:9–15
Radhakrishnan T, Murthy TGK, Chandran K, Bandyopadhyay A (2000) Micropropagation in peanut (Arachis hypogaea L.). Biol Plant 43(3):447–450
Matand K, Prakash CS (2007) Evaluation of peanut genotypes for in vitro plant regeneration using thidiazuron. J Biotechnol 130:202–207
Tiwari S, Tuli R (2009) Multiple shoot regeneration in seed-derived immature leaflet explants of peanut (Arachis hypogaea L.). Scientia Hortic 121:223–227
Shan L, Tang G, Xu P, Liu Z, Bi Y (2009) High efficiency in vitro plant regeneration from epicotyl explants of Chinese peanut cultivars. In Vitro Cell Dev Biol Plant 45:525–531
Durham RE, Parrott WA (1992) Repetitive somatic embryogenesis from peanut cultures in liquid medium. Plant Cell Rep 11:122–125
Eapen S, George L (1993) Somatic embryogenesis in peanut: influence of growth regulators and sugars. Plant Cell Tissue Org Cult 35:151–156
Baker CM, Wetzstein HY (1994) Influence of auxin type and concentration on peanut somatic embryogenesis. Plant Cell Tissue Org Cult 36:361–368
Chengalrayan K, Sathaye SS, Hazra S (1994) Somatic embryogenesis from mature embryo-derived leaflets of peanut (Arachis hypogaea L.). Plant Cell Rep 13:578–581
Murthy BNS, Saxena PK (1994) Somatic embryogenesis in peanut (Arachis hypogaea L.) stimulation of direct differentiation of somatic embryos by forchlorfenuron (CPPU). Plant Cell Rep 14:145–150
Baker CM, Wetzstein HY (1995) Repetetive embryogenesis in peanut cotyledon cultures by continual exposure to 2,4-D. Plant Cell Tissue Org Cult 40:249–254
McKently AH (1995) Effect of genotype on somatic embryogenesis from axes of mature peanut embryos. Plant Cell Tissue Org Cult 42:251–254
Baker CM, Durham RE, Burns JA, Parrott WA, Wetzstein HY (1995) High frequency somatic embryogenesis in peanut (Arachis hypogaea L.) using mature, dry seed. Plant Cell Rep 15:38–42
Chengalrayan K, Mhaske VB, Hazra S (1997) High-frequency conversion of abnormal peanut somatic embryos. Plant Cell Rep 16:783–786
Chengalrayan K, Mhaske VB, Hazra S (1998) Genotypic control of peanut somatic embryogenesis. Plant Cell Rep 17:522–525
Baker CM, Wetzstein HY (1998) Leaflet development, induction time, and medium influence somatic embryogenesis in peanut (Arachis hypogaea L.). Plant Cell Rep 17:925–929
Little EL, Magbanua ZV, Parrott WA (2000) A protocol for repetitive somatic embryogenesis from mature peanut epicotlys. Plant Cell Rep 19:351–357
Cucco MF, Jaume ADR (2000) Protocol for regeneration in vitro of Arachis hypogaea L. Electron J Biotechnol 3(2):154–160
Joshi MV, Sahasrabudhe NA, Hazra S (2003) Responses of peanut somatic embryos to thidiazuron. Biol Plant 46(2):187–192
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
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Ozudogru, E.A., Kaya, E., Lambardi, M. (2012). In Vitro Propagation of Peanut (Arachis hypogaea L.) by Shoot Tip Culture. In: Lambardi, M., Ozudogru, E., Jain, S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology, vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_6
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DOI: https://doi.org/10.1007/978-1-62703-074-8_6
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