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Protocols for Micropropagation of Selected Economically-Important Horticultural Plants pp 383–395Cite as

In Vitro Rejuvenation of Woody Species

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 994))

Abstract

Juvenility and phase change in woody plant species exert profound impacts on plant morphology and the ability of explants to be successfully propagated in vitro. Morphological characteristics such as leaf shape modifications, thorniness, and the inability to initiate flowers are associated with juvenility. Physiological maturity, that is the ability to reproduce sexually, is reached by many woody species only after many years of juvenile growth. As a result, micropropagation of woody species has historically been difficult with many plant species proving to be exceedingly recalcitrant. The importance of juvenility and its impact on successful vegetative reproduction in vitro has therefore received much research attention. In vitro technologies that have been demonstrated to induce rejuvenation include meristem culture, chemical treatments, pruning and hedging, forcing new growth, and taking advantage of epicormic buds, grafting and micrografting, and somatic embryogenesis. Applications of these technologies are discussed in this chapter.

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Authors and Affiliations

  1. Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, USA

    Paul E. Read

  2. Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA

    Christina M. Bavougian

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  1. Paul E. Read
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  2. Christina M. Bavougian
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Correspondence to Paul E. Read .

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Editors and Affiliations

  1. , IVALSA/Istituto per la Valorizzazione, National Research Council (CNR), Polo Scientifico, via Madonna del Piano n. 10, Sesto Fiorentino, Florence, 50019, Italy

    Maurizio Lambardi

  2. , Istituto per la Valorizzazione del Legno, National Research Council (CNR), n/a, Florence, n/a, Italy

    Elif Aylin Ozudogru

  3. , Department of Agricultural, University of Helsinki, 5 Latokartanonkaari Room C 130, Helsinki, 00014, Finland

    Shri Mohan Jain

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Read, P.E., Bavougian, C.M. (2012). In Vitro Rejuvenation of Woody Species. In: Lambardi, M., Ozudogru, E., Jain, S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology, vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_30

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