Abstract
Gerbera (Gerbera jamesonii Bolus) is one of the most popular ornamental flowers worldwide and used both as cut flower and potted plant. Some of them show excellent agronomic characters such as color, floral diameter, stem length, and vigor, which make this plant of commercial importance. Conventionally, multiplication is done through seeds or rhizome cuttings. Rapid multiplication of elite cultivars of Gerbera, with improved agronomic traits, has been achieved by using both direct and indirect tissue culture methods. Direct shoot regeneration was accomplished from stem apices on MS medium supplemented with 1 mg/L 6-benzyladenine (BA) and 1 mg/L kinetin. Indirect shoot induction succeeded from callus differentiation has been achieved on MS medium containing 2 mg/L 2,4-dichlorophenoxyacetic acid, 0.5 mg/L indole-3-acetic acid, and 2 mg/L BA. The in vitro shoots, 4–5 cm long, were rooted by quick dipping the shoot bases for 3–5 s in 2,000 mg/L indole-3-butyric acid solution followed by transfer to the pots containing farmyard manure, soil, and sand (1:1:1 by volume). Initially, in vitro plantlets were covered with glass jars to maintain a high relative humidity (85–90%). As soon as new shoot growth begins, relative humidity is decreased by exposing them to the open environmental conditions prior transferring to the glasshouse. Indirect shoot regeneration increased the frequency of somaclonal variations. The selected somaclones were used in developing new and novel cultivars.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Broek Van Den L, Haydu JJ, Hodges AW, Neves EM (2004) Production, marketing and distribution of cut flowers in US and Brazil. In: Annual report of Florida Agricultural Experiment Station, University of Florida, Gainesville, pp 1–19. http://hortbusiness.ifas.ufl.edu/cutUSDA/ERS%20Flowers%20Brazil-us.pdf
Parasarthy VA, Nagaraju V (1999) In vitro propagation of Gerbera jamesonii Bolus. Indian J Hortic 56:82–83
Aswath CR, Choudhary ML (2002) Rapid plant regeneration from Gerbera jamesonii Bolus callus cultures. Acta Bot Croat 61:125–134
Zhang W (2002) Research on rapid propagation of Gerbera jamesonii. Fujian Agric Sci Technol 1:17–18
Murashige T, Serpa M, Jones JR (1974) Clonal multiplication of gerbera through tissue culture. HortScience 9:175–180
Petru F, Matous J (1984) In vitro cultures of gerbera (Gerbera jamesonii Bolus). Shornik-UVTIZ Zahradnictvi 11:309–311
Huang MC, Chu CY (1985) A scheme for commercial multiplication of gerbera (Gerbera hybrida Hort.) through shoot tip culture. Jpn Soc Hortic Sci 54:94–100
Parthasarthy VA, Parthasarthy U, Nagaraju V, Mishara M (1997) Callus induction and subsequent plant regeneration from leaf explants of Gerbera jamesonii. Folia Hortic 9:83–86
Le CL, Julmi C, Thomas D, Tschuy F (1999) In vitro regeneration and multiplication of Gerbera jamesonii Bolus. Revue Suisse de Viticulture, d’ Arboriculture et d’Hort 31:207–211
Posada M, Ballesteros N, Obando W, Angarita A (1999) Micropropagation of gerbera from floral buds. Acta Hortic 482:329–332
Pierik RLM, Jansen JLN, Maasdan A, Binnendijk CM (1975) Optimization of gerbera plantlet production from excised capitulum explants. Sci Hortic 3:351–357
Chen F, Chen B (2002) Effect of La3+ on tissue culture of Gerbera jamesonii ‘Sunbird’. Acta Hortic 29:383–385
Laliberte S, Chretien I, Veith J (1985) In vitro plantlet production from young capitulum explants of Gerbera jamesonii. HortScience 20:137–139
Schum A, Busold M (1985) In vitro shoot production from inflorescence of Gerbera. Gb-t-Gw 85:1744–1746
Murashige T, Skoog F (1962) Revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–479
Kumar S, Kanwar JK, Sharma DR (2004) In vitro regeneration of Gerbera jamesonii Bolus from leaf and petiole explants. J Biochem Biotechnol 13:73–75
Kumar S, Kanwar JK (2005) Plant regeneration from callus and cell suspension cultures of Gerbera jamesonii cv. Diablo. Eur J Hortic Sci 70:265–270
Kumar S, Kanwar JK (2006) Regeneration ability of petiole, leaf and petal explants in gerbera cut flower cultures in vitro. Folia Hortic 18:57–64
Reynoird JP, Chriqui D, Noin M, Brown S, Marie D (1993) Plant propagation from in vitro leaf culture of several Gerbera species. Plant Cell Tissue Organ Cult 33:203–210
Ray T, Saha P, Roy SC (2005) In vitro plant regeneration from young capitulum explants of Gerbera jamesonii. Plant Cell Biotechnol Mol Biol 6:35–40
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media New York
About this protocol
Cite this protocol
Minerva, G., Kumar, S. (2012). Micropropagation of Gerbera (Gerbera jamesonii Bolus). In: Lambardi, M., Ozudogru, E., Jain, S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology, vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_24
Download citation
DOI: https://doi.org/10.1007/978-1-62703-074-8_24
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-073-1
Online ISBN: 978-1-62703-074-8
eBook Packages: Springer Protocols