Abstract
Induction of somatic embryogenesis is described in common ash (Fraxinus excelsior L.). Embryogenic tissues are obtained from immature zygotic embryos and cultured on a modified Murashige and Skoog (MS) medium containing 8.8 μM 2,4-dichlorophenoxyacetic acid and 4.4 μM benzyl-adenine. Embryogenic tissue is subcultured and multiplied on medium supplemented with reduced concentration of plant growth hormones. Somatic embryos develop and mature by transfer to hormone-free medium and subsequent culture on medium containing low amount of benzyladenine. Somatic embryo germination and conversion are enhanced by cold storage at 4°C and successive transfer onto Woody Plant Medium (WPM). Fully developed plantlets are then transferred to pots and acclimatized in the greenhouse equipped with a mist system.
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Capuana, M. (2012). In Vitro Propagation of Ash (Fraxinus excelsior L.) by Somatic Embryogenesis. In: Lambardi, M., Ozudogru, E., Jain, S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology, vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_16
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DOI: https://doi.org/10.1007/978-1-62703-074-8_16
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