Abstract
Lavandula species are some of the most popular ornamental and medicinal plants with great economic values. These species are vegetative propagated by stem cuttings. However, the poor rooting ability and vulnerability of plantlets to contamination are major limiting factors for propagation. In vitro culture methods are suitable to overcome these limitations. This chapter describes protocols for in vitro propagation of Lavandula viridis L’Hér and Lavandula vera DC. Nodal shoot proliferation of L. viridis and plant regeneration from leaf-derived callus of L. vera by an “open culture system” are highlighted.
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References
Hanamanthagouda MS, Kakkalameli SB, Naik PM, Nagella P, Seetharamareddy HR, Murthy HN (2010) Essential oils of Lavandula bipinnata and their antimicrobial activities. Food Chem 118:836–839
Falk L, Biswas K, Boeckelmann A, Lane A, Mahmoud SS (2009) An efficient method for the micropropagation of Lavenders: regeneration of a unique mutant. J Essent Oil Res 21:225–228
Pavela R (2005) Insecticidal activity of some essential oils against larvae of Spodoptera littoralis. Fitoterapia 76:691–696
Haig TJ, Haig TJ, Seal AN, Pratley JE, An M, Wu H (2009) Lavender as a source of novel plant compounds for the development of a natural herbicide. J Chem Ecol 35:1129–1136
Panizza M, Tognoni F (1992) Micropropagation of lavandin (Lavandula officinalis Chaix × Lavandula latifolia Villars cv. Grosso). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, high-tech and micropropagation III, vol 19. Springer, Berlin
Sánchez-Gras C, Calvo MC (1996) Micropropagation of Lavandula latifolia through nodal bud culture of mature plants. Plant Cell Tissue Organ Cult 45:259–261
Nobre J (1996) In vitro cloning and micropropagation of Lavandula stoechas from field-grown plants. Plant Cell Tissue Organ Cult 46:151–155
Echeverrigaray S, Basso R, Andrade LB (2005) Micropropagation of Lavandula dentata from axillary buds of field-grown adult plants. Biol Plant 49:439–442
Dronne S, Jullien F, Caissard J-C, Faure O (1999) A simple and efficient method for in vitro shoot regeneration from leaves of lavandin (Lavandula × intermedia Emeric ex Loiseleur). Plant Cell Rep 18:429–433
Calvo MC, Segura J (1989) In vitro propagation of Lavender. Hortscience 24:375–376
Jordan AM, Calvo MC, Segura J (1998) Micropropagation of adult Lavandula dentata plants. J Hortic Sci Biotechnol 73:93–96
Dias MC, Almeida R, Romano A (2002) Rapid clonal multiplication of Lavandula viridis L’Hér through in vitro axillary shoot proliferation. Plant Cell Tissue Organ Cult 68:99–102
Andrade LB, Echeverrigaray S, Fracaro F, Pauletti GF, Rota L (1999) The effect of growth regulators on shoot propagation and rootin g of common lavender (Lavandula vera DC). Plant Cell Tissue Organ Cult 56:79–83
Ghiorghiţă G, Maftei D-E, Nicuţă D (2009) Some aspects concerning the in vitro reaction of Lavandula angustifolia L. Propag Ornam Plants 9:47–49
Tsuro M, Koda M, Inoue M (1999) Comparative effect of different types of cytokinin for shoot formation and plant regeneration in leaf-derived callus of lavender (Lavandula vera DC). Sci Hortic 81:331–336
Tsuro M, Koda M, Inoue M (2000) Efficient plant regeneration from multiple shoots formed in the leaf-derived callus of Lavandula vera, using the “open culture system”. Sci Hortic 86:81–88
Wang X, Jin L, Li M, Zhao M, Zhao M, Zhao H, Xu Y (2007) Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, Lavandula angustifolia ‘Munstead’. J Hortic Sci Biotechnol 82:781–785
Nogueira JMF, Romano A (2002) Essential oils from micropropagated plants of Lavandula viridis. Phytochem Anal 13:4–7
Gonçalves S, Serra H, Nogueira JMF, Almeida R, Custódio L, Romano A (2008) Headspace-SPME of in vitro shoot-cultures and micropropagated plants of Lavandula viridis. Biol Plant 52:133–136
Shellie R, Mondello L, Marriott P, Dugo G (2002) Characterisation of lavender essential oils by using gas chromatography–mass spectrometry with correlation of linear retention indices and comparison with comprehensive two-dimensional gas chromatography. J Chromatogr A 970:225–234
Bradley BF, Starkey NJ, Browna SL, Lea RW (2007) Anxiolytic effects of Lavandula angustifolia odour on the Mongolian gerbil elevated plus maze. J Ethnopharmacol 111:517–525
McGimpsey JA, Porter NG (1999) Lavender. A growers guide for commercial production. New Zealand Institute for Crop and Food Research Limited, Christchurch, New Zealand
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Gresshoff P, Doy C (1972) Development and differentiation of haploid Lycopersicon esculentum (Tomato). Planta 170:161–170
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Gonçalves, S., Romano, A. (2012). Micropropagation of Lavandula spp.. In: Lambardi, M., Ozudogru, E., Jain, S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology, vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_14
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DOI: https://doi.org/10.1007/978-1-62703-074-8_14
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