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Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells

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Cell Imaging Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 931))

Abstract

One of the challenging tasks in molecular cell biology is to identify and localize specific binding sites on biological samples with high spatial accuracy (in order of several nm). During the past 5 years, simultaneous topography and recognition imaging (TREC) has become a powerful AFM-based technique for quick and easy high-resolution receptor mapping. In this chapter, we provide a flavor of TREC application on vascular endothelial cells by describing the detailed procedures for all stages of the experiment from tip and sample preparations through the operating principles and visualization.

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Author information

Authors and Affiliations

  1. Center for Advanced Bioanalysis (CBL GmbH), Linz, Austria

    Lilia A. Chtcheglova & Peter Hinterdorfer

  2. Institute for Biophysics, University of Linz, Linz, Austria

    Peter Hinterdorfer

Authors
  1. Lilia A. Chtcheglova
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  2. Peter Hinterdorfer
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Corresponding author

Correspondence to Lilia A. Chtcheglova .

Editor information

Editors and Affiliations

  1. College of Medicine, Professor, Department of Pathology, University of Vermont, Beaumont Ave 89, Burlington, 05405-1742, Vermont, USA

    Douglas J. Taatjes

  2. , Abt. Zell- und Molekularpathologie, Universität Zürich, Schmelzbergstr. 12, Zürich, 8091, Switzerland

    Jürgen Roth

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Chtcheglova, L.A., Hinterdorfer, P. (2012). Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells. In: Taatjes, D., Roth, J. (eds) Cell Imaging Techniques. Methods in Molecular Biology, vol 931. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-056-4_16

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  • DOI: https://doi.org/10.1007/978-1-62703-056-4_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-055-7

  • Online ISBN: 978-1-62703-056-4

  • eBook Packages: Springer Protocols

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