The Hemizona Assay for Assessment of Sperm Function
The hemizona assay (HZA) has been developed as a diagnostic test for the tight binding of human spermatozoa to the human zona pellucida to predict fertilization potential. In this homologous bioassay, the two matching hemizona halves are functionally equal surfaces allowing controlled comparison of binding from a fertile control versus a test sample, with reproducible measurements of sperm binding obtained from a single oocyte. Oocytes from different sources (surplus from IVF treatment or recovered from ovarian tissue) are salt-stored and used after microbisection. Extensive clinical data have demonstrated excellent predictive power of the HZA for the outcomes of intrauterine insemination and IVF, and therefore the assay has relevance in the clinical diagnostic setting in infertility.
Key wordsHemizona assay Oocyte Sperm function
- 29.(1996) Consensus workshop on advanced diagnostic andrology techniques. ESHRE (European Society of Human Reproduction and Embryology) Andrology Special Interest Group. Hum Reprod 11: 1463–1479Google Scholar
- 30.World Health Organization (2010) World Health Organization laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge University Press, Cambridge, UK, pp 31–33Google Scholar