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The Hemizona Assay for Assessment of Sperm Function

  • Sergio Oehninger
  • Mahmood Morshedi
  • Daniel Franken
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 927)

Abstract

The hemizona assay (HZA) has been developed as a diagnostic test for the tight binding of human spermatozoa to the human zona pellucida to predict fertilization potential. In this homologous bioassay, the two matching hemizona halves are functionally equal surfaces allowing controlled comparison of binding from a fertile control versus a test sample, with reproducible measurements of sperm binding obtained from a single oocyte. Oocytes from different sources (surplus from IVF treatment or recovered from ovarian tissue) are salt-stored and used after microbisection. Extensive clinical data have demonstrated excellent predictive power of the HZA for the outcomes of intrauterine insemination and IVF, and therefore the assay has relevance in the clinical diagnostic setting in infertility.

Key words

Hemizona assay Oocyte Sperm function 

References

  1. 1.
    Oehninger S et al (1997) Clinical significance of human sperm-zona pellucida binding. Fertil Steril 67:1121–1127PubMedCrossRefGoogle Scholar
  2. 2.
    Oehninger S et al (1997) Approaching the next millennium: how should we manage andrology diagnosis in the intracytoplasmic sperm injection era? Fertil Steril 67:434–436PubMedCrossRefGoogle Scholar
  3. 3.
    Oehninger S (1995) An update on the laboratory assessment of male fertility. Hum Reprod 10(Suppl 1):38–45PubMedGoogle Scholar
  4. 4.
    Franken DR, Oehninger S (2006) The clinical significance of sperm-zona pellucida binding: 17 years later. Front Biosci 11:1227–1233PubMedCrossRefGoogle Scholar
  5. 5.
    Oehninger S (2011) Clinical management of male infertility in assisted reproduction: ICSI and beyond. Int J Androl 34:e319–e329PubMedCrossRefGoogle Scholar
  6. 6.
    Burkman LJ et al (1988) The hemizona assay (HZA): development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential. Fertil Steril 49:688–697PubMedGoogle Scholar
  7. 7.
    Hodgen GD et al (1988) The hemizona assay (HZA): finding sperm that have the “right stuff”. J In Vitro Fert Embryo Transf 5:311–313PubMedCrossRefGoogle Scholar
  8. 8.
    Overstreet JW, Hembree WC (1976) Penetration of the zona pellucida of nonliving human oocytes by human spermatozoa in vitro. Fertil Steril 27:815–831PubMedGoogle Scholar
  9. 9.
    Yanagimachi R et al (1979) Retention of biologic characteristics of zona pellucida in highly concentrated salt solution: the use of salt-stored eggs for assessing the fertilizing capacity of spermatozoa. Fertil Steril 31:562–574PubMedGoogle Scholar
  10. 10.
    Franken DR et al (1989) Hemizona assay using salt-stored human oocytes: evaluation of zona pellucida capacity for binding human spermatozoa. Gamete Res 22:15–26PubMedCrossRefGoogle Scholar
  11. 11.
    Franken DR et al (1989) The hemizona assay (HZA): a predictor of human sperm fertilizing potential in in vitro fertilization (IVF) treatment. J In Vitro Fert Embryo Transf 6:44–50PubMedCrossRefGoogle Scholar
  12. 12.
    Franken DR et al (1991) Defining the valid hemizona assay: accounting for binding variability within zonae pellucidae and within semen samples from fertile males. Fertil Steril 56:1156–1161PubMedGoogle Scholar
  13. 13.
    Franken DR et al (1991) Comparison of sperm binding potential of uninseminated, inseminated-unfertilized, and fertilized-noncleaved human oocytes under hemizona assay conditions. Mol Reprod Dev 30:56–61PubMedCrossRefGoogle Scholar
  14. 14.
    Franken DR et al (1994) Sperm binding capacity of human zona pellucida derived from oocytes obtained from different sources. Andrologia 26:277–281PubMedCrossRefGoogle Scholar
  15. 15.
    Coddington CC et al (1990) Sperm bound to zona pellucida in hemizona assay demonstrate acrosome reaction when stained with T-6 antibody. Fertil Steril 54:504–508PubMedGoogle Scholar
  16. 16.
    Oehninger S et al (1989) Hemizona assay: assessment of sperm dysfunction and prediction of in vitro fertilization outcome. Fertil Steril 51:665–670PubMedGoogle Scholar
  17. 17.
    Oehninger S et al (1991) Human preovulatory oocytes have a higher sperm-binding ability than immature oocytes under hemizona assay conditions: evidence supporting the concept of “zona maturation”. Fertil Steril 55:1165–1170PubMedGoogle Scholar
  18. 18.
    Bastiaan HS et al (2003) Relationship between zona pellucida-induced acrosome reaction, sperm morphology, sperm-zona pellucida binding, and in vitro fertilization. Fertil Steril 79:49–55PubMedCrossRefGoogle Scholar
  19. 19.
    Oehninger S et al (1989) Validation of the hemizona assay in a monkey model: influence of oocyte maturational stages. Fertil Steril 51:881–885PubMedGoogle Scholar
  20. 20.
    Franken DR et al (1991) Electron microscopic evidence on the acrosomal status of bound sperm and their penetration into human hemizonae pellucida after storage in a buffered salt solution. Andrologia 23:205–208PubMedCrossRefGoogle Scholar
  21. 21.
    Oehninger S et al (1993) Validation of the hemizona assay (HZA) in a monkey model. II. Kinetics of binding and influence of cryopreserved-thawed spermatozoa. J Assist Reprod Genet 10:292–301PubMedCrossRefGoogle Scholar
  22. 22.
    Oehninger S et al (1993) The specificity of human spermatozoa/zona pellucida interaction under hemizona assay conditions. Mol Reprod Dev 35:57–61PubMedCrossRefGoogle Scholar
  23. 23.
    Franken DR et al (1993) The ability of the hemizona assay to predict human fertilization in different and consecutive in-vitro fertilization cycles. Hum Reprod 8:1240–1244PubMedGoogle Scholar
  24. 24.
    Oehninger S et al (1991) Recurrent failure of in vitro fertilization: role of the hemizona assay in the sequential diagnosis of specific sperm-oocyte defects. Am J Obstet Gynecol 164:1210–1215PubMedGoogle Scholar
  25. 25.
    Oehninger S et al (1991) Hemizona assay: sperm defect analysis, a diagnostic method for assessment of human sperm-oocyte interactions, and predictive value for fertilization outcome. Ann N Y Acad Sci 626:111–124PubMedCrossRefGoogle Scholar
  26. 26.
    Oehninger S et al (1992) Hemizona assay and its impact on the identification and treatment of human sperm dysfunctions. Andrologia 24:307–321PubMedCrossRefGoogle Scholar
  27. 27.
    Oehninger S et al (2000) Sperm function assays and their predictive value for fertilization outcome in IVF therapy: a meta-analysis. Hum Reprod Update 6:160–168PubMedCrossRefGoogle Scholar
  28. 28.
    Arslan M et al (2006) Predictive value of the hemizona assay for pregnancy outcome in patients undergoing controlled ovarian hyperstimulation with intrauterine insemination. Fertil Steril 85:1697–1707PubMedCrossRefGoogle Scholar
  29. 29.
    (1996) Consensus workshop on advanced diagnostic andrology techniques. ESHRE (European Society of Human Reproduction and Embryology) Andrology Special Interest Group. Hum Reprod 11: 1463–1479Google Scholar
  30. 30.
    World Health Organization (2010) World Health Organization laboratory manual for the examination of human semen and sperm-­cervical mucus interaction. Cambridge University Press, Cambridge, UK, pp 31–33Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Sergio Oehninger
    • 1
  • Mahmood Morshedi
    • 1
  • Daniel Franken
    • 2
  1. 1.The Jones Institute for Reproductive MedicineEastern Virginia Medical SchoolNorfolkUSA
  2. 2.Reproductive Biology Unit, Tygerberg HospitalUniversity of StellenboschCape TownRSA

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