The Hemizona Assay for Assessment of Sperm Function

  • Sergio OehningerEmail author
  • Mahmood Morshedi
  • Daniel Franken
Part of the Methods in Molecular Biology book series (MIMB, volume 927)


The hemizona assay (HZA) has been developed as a diagnostic test for the tight binding of human spermatozoa to the human zona pellucida to predict fertilization potential. In this homologous bioassay, the two matching hemizona halves are functionally equal surfaces allowing controlled comparison of binding from a fertile control versus a test sample, with reproducible measurements of sperm binding obtained from a single oocyte. Oocytes from different sources (surplus from IVF treatment or recovered from ovarian tissue) are salt-stored and used after microbisection. Extensive clinical data have demonstrated excellent predictive power of the HZA for the outcomes of intrauterine insemination and IVF, and therefore the assay has relevance in the clinical diagnostic setting in infertility.

Key words

Hemizona assay Oocyte Sperm function 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Sergio Oehninger
    • 1
    Email author
  • Mahmood Morshedi
    • 1
  • Daniel Franken
    • 2
  1. 1.The Jones Institute for Reproductive MedicineEastern Virginia Medical SchoolNorfolkUSA
  2. 2.Reproductive Biology Unit, Tygerberg HospitalUniversity of StellenboschCape TownRSA

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