Methods of Sperm Vitality Assessment

  • Sergey I. Moskovtsev
  • Clifford L. Librach
Part of the Methods in Molecular Biology book series (MIMB, volume 927)


Sperm vitality is a reflection of the proportion of live, membrane-intact spermatozoa determined by either dye exclusion or osmoregulatory capacity under hypo-osmotic conditions. In this chapter we address the two most common methods of sperm vitality assessment: eosin–nigrosin staining and the hypo-osmotic swelling test, both utilized in clinical Andrology laboratories.

Key words

Sperm vitality Eosin–nigrosin staining Hypo-osmotic swelling 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.CReATe Fertility CenterTorontoCanada
  2. 2.Department of Obstetrics & GynaecologyUniversity of TorontoTorontoCanada
  3. 3.Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynaecology, CReATe Fertility Center, Sunnybrook Health Sciences Centre and Women’s College HospitalUniversity of TorontoTorontoCanada

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