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Methods of Sperm Vitality Assessment

  • Sergey I. Moskovtsev
  • Clifford L. Librach
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 927)

Abstract

Sperm vitality is a reflection of the proportion of live, membrane-intact spermatozoa determined by either dye exclusion or osmoregulatory capacity under hypo-osmotic conditions. In this chapter we address the two most common methods of sperm vitality assessment: eosin–nigrosin staining and the hypo-osmotic swelling test, both utilized in clinical Andrology laboratories.

Key words

Sperm vitality Eosin–nigrosin staining Hypo-osmotic swelling 

References

  1. 1.
    WHO (2010) World Health Organization laboratory manual for the examination and processing of human semen. WHO Press, GenevaGoogle Scholar
  2. 2.
    Chemes EH, Rawe YV (2003) Sperm pathology: a step beyond descriptive morphology. Origin, characterization and fertility potential of abnormal sperm phenotypes in infertile men. Hum Reprod Update 9:405–428PubMedCrossRefGoogle Scholar
  3. 3.
    Mortimer D (1994) Practical laboratory andrology. Oxford University Press, New YorkGoogle Scholar
  4. 4.
    Gillan L et al (2005) Flow cytometric evaluation of sperm parameters in relation to fertility potential. Theriogenology 63:445–457PubMedCrossRefGoogle Scholar
  5. 5.
    Jeyendran RS et al (1984) Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 70:219–228PubMedCrossRefGoogle Scholar
  6. 6.
    Munuce MJ et al (2000) Does the hypoosmotic swelling test predict human sperm viability? Arch Androl 44:207–212PubMedCrossRefGoogle Scholar
  7. 7.
    Hossain AM et al (1998) Time course of hypo-osmotic swellings of human spermatozoa: evidence of ordered transition between swelling subtypes. Hum Reprod 13:1578–1583PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.CReATe Fertility CenterTorontoCanada
  2. 2.Department of Obstetrics & GynaecologyUniversity of TorontoTorontoCanada
  3. 3.Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynaecology, CReATe Fertility Center, Sunnybrook Health Sciences Centre and Women’s College HospitalUniversity of TorontoTorontoCanada

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