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Spermatogenesis pp 217–226Cite as

Density Gradient Separation of Sperm for Artificial Insemination

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 927))

Abstract

Human spermatozoa for clinical procedures such as IUI or IVF, or for diagnostic or research studies of sperm fertilizing ability, must be separated from the seminal plasma environment not only as soon as possible after ejaculation but also as efficiently as possible, minimizing seminal plasma and bacterial carryover. Furthermore, in addition to technical simplicity and robustness, a sperm preparation method needs to select not just the more motile and morphologically normal spermatozoa but also those spermatozoa with reduced DNA damage. Currently the most effective and efficient technique for this is density gradient centrifugation, which has been extensively validated through research and clinical application. An optimized protocol based on silane-coated colloidal silica products is provided.

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Author information

Authors and Affiliations

  1. Oozoa Biomedical Inc., West Vancouver, BC, Canada

    David Mortimer & Sharon T. Mortimer

Authors
  1. David Mortimer
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  2. Sharon T. Mortimer
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Corresponding author

Correspondence to David Mortimer .

Editor information

Editors and Affiliations

  1. Andrology and IVF Laboratories, Department of Surgery (Urology), University of Utah School of Medicine, Arapeen Dr. 675, Salt Lake City, 84108, Utah, USA

    Douglas T. Carrell

  2. , Andrology and IVF Laboratories, University of Utah School of Medicine, Arapeen Way 675, Salt Lake City, 84108, Utah, USA

    Kenneth I. Aston

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Mortimer, D., Mortimer, S.T. (2013). Density Gradient Separation of Sperm for Artificial Insemination. In: Carrell, D., Aston, K. (eds) Spermatogenesis. Methods in Molecular Biology, vol 927. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-038-0_19

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  • DOI: https://doi.org/10.1007/978-1-62703-038-0_19

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-037-3

  • Online ISBN: 978-1-62703-038-0

  • eBook Packages: Springer Protocols

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