Abstract
Precise, fast, and cheap identification of pathogenic bacteria is a key factor of microbial diagnostics. Application of analytical techniques can provide new possibilities in this regard. The bacterial pathogen Staphylococcus aureus is responsible for a number of serious human infections which are hard for eradication since the number of multidrug resistant isolates is increasing. In the present chapter, we describe novel methods based on capillary zone electrophoresis (CZE) and (as confirmation of these results) molecular analysis of a part of the coag gene for identification and differentiation of S. aureus strains.
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References
Weems JJ (2001) The many faces of Staphylococcus aureus infection. Recognizing and managing its life-threatening manifestations. Postgrad Med 110:24–36
Reniere ML, Victor JT, Skaar EP (2007) Intracellular metaloporphyrin metabolism in Staphylococcus aureus. Biometals 20:333–345
Buszewski B et al (2003) Separation of bacteria by capillary electrophoresis. J Sep Sci 26:1045–1049
Armstrong DW, Schneiderheinze JM (2000) Rapid identification of the bacterial pathogens responsible for urinary tract infections using direct injection CE. Anal Chem 72:4474–4476
Moon BG et al (2003) Capillary electrophoresis of microbes. Bull Kor Chem Soc 24:81–85
Palenzuela B et al (2004) Monitoring of bacterial contamination in food samples using capillary zone electrophoresis. Anal Chem 76:3012–3017
Lim O, Suntornsuk W, Suntornsuk L (2009) Capillary zone electrophoresis for enumeration of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus in yogurt. J Chromatogr B 877:710–718
Buszewski B et al (2007) Rapid identification of Helicobacter pylori by capillary electrophoresis: an overview. Biomed Chromatogr 21:116–122
Kłodzińska E et al (2009) S. aureus strains differentiation by electrophoresis and zeta potential measurements combined with phylogenetic relationship based on coagulase gene and protein polymorphism. Electrophoresis 30:3086–3091
Kłodzińska E et al (2010) Effect of zeta potential value on bacterial behavior during electrophoretic separation. Electrophoresis 31:1590–1596
Szumski M, Kłodzińska E, Buszewski B (2005) Separation of microorganisms using electromigration techniques. J Chromatogr A 1084:186–193
Goh SH et al (1992) Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms. J Clin Microbiol 30:1642–1645
Hrynkiewicz K et al (2008) Combination of capillary electrophoresis, PCR and physiological assays in differentiation of clinical strains of Staphylococcus aureus. FEMS Microbiol Lett 286:1–8
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Buszewski, B., Hrynkiewicz, K., Dziubakiewicz, E. (2013). Application of CZE in the Differentiation of Staphylococcus aureus Strains. In: Phillips, T., Kalish, H. (eds) Clinical Applications of Capillary Electrophoresis. Methods in Molecular Biology, vol 919. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-029-8_14
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DOI: https://doi.org/10.1007/978-1-62703-029-8_14
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-028-1
Online ISBN: 978-1-62703-029-8
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