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Solution NMR Studies of Integral Polytopic α-Helical Membrane Proteins: The Structure Determination of the Seven-Helix Transmembrane Receptor Sensory Rhodopsin II, pSRII

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Membrane Protein Structure and Dynamics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 914))

Abstract

About 30% of the proteins encoded in the genome are expressed as membrane proteins but these represent <1% of all the structures solved today. In view of the physiological and pharmaceutical significance of membrane proteins it is clear that a better and more comprehensive understanding of their three-dimensional (3D) structures at atomic resolution is required. α-Helical integral membrane proteins are generally more difficult to work with than β-barrel-type proteins and this has particularly been true for the polytopic members such as the large family of seven-helical proteins. In this chapter we describe the practical aspects of the solution-state NMR spectroscopy structure determination of the seven-helical transmembrane (7-TM) protein receptor sensory rhodopsin pSRII from the haloalkaliphilic archaeon Natronomonas pharaonis reconstituted in detergent micelles. This is the first time that a three-dimensional structure of a 7-TM protein has been determined by NMR.

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Authors and Affiliations

  1. Department of Biochemistry, University of Cambridge, Cambridge, UK

    Antoine Gautier & Daniel Nietlispach

Authors
  1. Antoine Gautier
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  2. Daniel Nietlispach
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Corresponding author

Correspondence to Daniel Nietlispach .

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Editors and Affiliations

  1. , Division of Immunology, Beckman Research Institute of the City o, E. Duarte Road 1500, Duarte, 91010, USA

    Nagarajan Vaidehi

  2. , Department of Structural Biology, University of Pittsburgh School of Medic, Fifth Avenue 3501, Pittsburgh, 15261, USA

    Judith Klein-Seetharaman

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Gautier, A., Nietlispach, D. (2012). Solution NMR Studies of Integral Polytopic α-Helical Membrane Proteins: The Structure Determination of the Seven-Helix Transmembrane Receptor Sensory Rhodopsin II, pSRII. In: Vaidehi, N., Klein-Seetharaman, J. (eds) Membrane Protein Structure and Dynamics. Methods in Molecular Biology, vol 914. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-023-6_3

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  • DOI: https://doi.org/10.1007/978-1-62703-023-6_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-022-9

  • Online ISBN: 978-1-62703-023-6

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