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Genomic Imprinting pp 61–66Cite as

Immunomagnetic Purification of Murine Primordial Germ Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 925))

Abstract

Primordial germ cells (PGCs) play essential roles in both reproduction and development. In this chapter, we describe a method used in our laboratory for the immunopurification of PGCs from the mouse embryo. After dissection and disruption of the fetal gonad, PGCs are identified by a monoclonal antibody recognizing an epitope characteristic of pluripotent stem cells. After reaction with a paramagnetic bead-linked secondary antibody, the cell mixture is applied to a strong magnetic field. PGCs are recovered by release from the magnetic field. Purity is assessed by the alkaline phosphatase activity inherent to PGCs.

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Author information

Authors and Affiliations

  1. Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, FL, USA

    Emily Y. Smith & James L. Resnick

Authors
  1. Emily Y. Smith
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  2. James L. Resnick
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Corresponding author

Correspondence to James L. Resnick .

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Editors and Affiliations

  1. School of Medicine, Fels Institute, Temple University, N. Broad Street 3307, Philadelphia, 19140, Pennsylvania, USA

    Nora Engel

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Smith, E.Y., Resnick, J.L. (2012). Immunomagnetic Purification of Murine Primordial Germ Cells. In: Engel, N. (eds) Genomic Imprinting. Methods in Molecular Biology, vol 925. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-011-3_4

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  • DOI: https://doi.org/10.1007/978-1-62703-011-3_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-010-6

  • Online ISBN: 978-1-62703-011-3

  • eBook Packages: Springer Protocols

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