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Co-Immunoprecipitation of Long Noncoding RNAs

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Book cover Genomic Imprinting

Part of the book series: Methods in Molecular Biology ((MIMB,volume 925))

Abstract

It is now estimated that the human genome encodes thousands of long noncoding (lnc)RNAs. These novel molecules are causing a paradigm shift in the field of molecular biology as a number of lncRNAs have been shown to be involved in a wide range of biological functions including regulation of gene expression. Also, misregulation of lncRNAs has been observed in human diseases such as cancer and neurological disorders. These findings have spurred a huge interest in elucidating the functions and mechanisms of lncRNAs; and therefore, the need for new methods to do so. In this chapter, we discuss RIP-Seq, a method that is utilized to discover the lncRNA partners of a specific protein. This procedure involves immunoprecipitation of a protein from cross-linked cell lysate followed by reverse-cross-linking, isolation, and deep sequencing of RNAs, leading to the identification of all lncRNAs that are associated with a specific protein complex.

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Author information

Authors and Affiliations

  1. Department of Genetics, Center for RNA Molecular Biology, Case Western Reserve University School of Medicine, Cleveland, OH, USA

    Victoria A. Moran, Courtney N. Niland & Ahmad M. Khalil

Authors
  1. Victoria A. Moran
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  2. Courtney N. Niland
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  3. Ahmad M. Khalil
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Corresponding author

Correspondence to Ahmad M. Khalil .

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Editors and Affiliations

  1. School of Medicine, Fels Institute, Temple University, N. Broad Street 3307, Philadelphia, 19140, Pennsylvania, USA

    Nora Engel

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Moran, V.A., Niland, C.N., Khalil, A.M. (2012). Co-Immunoprecipitation of Long Noncoding RNAs. In: Engel, N. (eds) Genomic Imprinting. Methods in Molecular Biology, vol 925. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-011-3_15

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  • DOI: https://doi.org/10.1007/978-1-62703-011-3_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-010-6

  • Online ISBN: 978-1-62703-011-3

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