Genetic Manipulation of a Naturally Competent Bacterium, Helicobacter pylori

Noto, Jennifer M.; Peek, Richard M.

doi:10.1007/978-1-62703-005-2_8

Jennifer M. Noto² &
Richard M. Peek Jr.³

Part of the book series: Methods in Molecular Biology ((MIMB,volume 921))

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Abstract

Genetic manipulation of Helicobacter pylori facilitates characterization and functional analysis of individual H. pylori genes. This chapter discusses the methods involved in H. pylori chromosomal DNA isolation, mutagenesis of individual genes, and natural transformation.

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Authors and Affiliations

Division of Gastroenterology, Department of Medicine, Vanderbilt University Medical Center, MRB IV 1030C MRB IV, Nashville, TN, USA
Jennifer M. Noto
Division of Gastroenterology, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA
Richard M. Peek Jr.

Authors

Jennifer M. Noto
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Richard M. Peek Jr.
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Corresponding author

Correspondence to Richard M. Peek Jr. .

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Editors and Affiliations

Medical School, Dept. Medicine, University of Massachusetts, Plantation Street 364, Worcester, 01635, Massachusetts, USA
JeanMarie Houghton

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Noto, J.M., Peek, R.M. (2012). Genetic Manipulation of a Naturally Competent Bacterium, Helicobacter pylori . In: Houghton, J. (eds) Helicobacter Species. Methods in Molecular Biology, vol 921. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-005-2_8

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DOI: https://doi.org/10.1007/978-1-62703-005-2_8
Published: 23 August 2012
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-004-5
Online ISBN: 978-1-62703-005-2
eBook Packages: Springer Protocols

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