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Deriving TC50 Values of Nanoparticles from Electrochemical Monitoring of Lactate Dehydrogenase Activity Indirectly

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Nanotoxicity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 926))

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Abstract

Nanotoxicity assessment methods for nanoparticles (NPs) such as carbon nanotubes (CNTs), nano-Al2O3, and tridecameric aluminum polycation or nanopolynuclear (nano-Al13), particularly lactate dehydrogenase (LDH) assays are reviewed. Our researches on electrochemically monitoring the variations of LDH activity indirectly in the presence of multiwalled carbon nanotubes (MWCNTs), nano-Al13, and nano-Al2O3 separately to derive toxic concentrations of NPs altering LDH activity by 50% (TC50) values are discussed. TC50 values indicated that the toxicity order was Al (III)> MWCNTs > nano-Al13 > nano-Al2O3. Zeta potentials (ΞΆ) data of these NPs in the literature proved that the surfaces of these NPs are charged negatively. Negatively charged surfaces might be a main cause in the reduction of LDH activity. Therefore, the classic LDH assays are doubtful to underestimate the nanotoxicities when they are applied to those NPs with negatively charged surfaces. These observations highlight and reconcile some contradictory results at present such as medium-dependent toxicity of NPs among the literature and develop novel analytical methods for evaluation of toxicities of NPs.

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Acknowledgments

This project is supported by the NSFC (20975049), State Key Laboratory of Electrochemistry of China in Changchun Applied Chemistry Institute (2008008) and Analytical Center of Nanjing University.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination, Chemistry of China and MOE Key Laboratory for Life Science, Nanjing University, Nanjing, China

    Fuping Zhang,Β Na WangΒ &Β Fang Chang

  2. School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry of China and MOE Key Laboratory for Life Science, Nanjing University, Nanjing, China

    Shuping Bi

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  1. Fuping Zhang
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  2. Na Wang
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Correspondence to Shuping Bi .

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  1. College of Pharmacy & Health Sciences, Dept. of Pharmaceutical Sciences, Wayne State University, Mack Avenue 259, Detroit, 48201, Michigan, USA

    Joshua Reineke

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Zhang, F., Wang, N., Chang, F., Bi, S. (2012). Deriving TC50 Values of Nanoparticles from Electrochemical Monitoring of Lactate Dehydrogenase Activity Indirectly. In: Reineke, J. (eds) Nanotoxicity. Methods in Molecular Biology, vol 926. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-002-1_8

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  • DOI: https://doi.org/10.1007/978-1-62703-002-1_8

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