Abstract
Increasing application of silver nanoparticles (SNPs) and zinc oxide nanoparticles (nZnO) in consumer products like textiles, cosmetics, washing machines and other household products increases their chance to reach the environment. Intensive research is required to assess the nanoparticles’ toxicity to the environmental system. The toxicological effect of nanoparticles has been studied at the miniscule scale and requires intensive research to be conducted to assess its unknown effects. Plants are the primary target species which need to be included to develop a comprehensive toxicity profile for nanoparticles. So far, the mechanisms of toxicity of nanoparticles to the plant system remains largely unknown and little information on the potential uptake of nanoparticles by plants and their subsequent fate within the food chain is available. The phytoxicological behaviour of silver and zinc oxide nanoparticles on Allium cepa and seeds of Zea mays (maize), Cucumis sativus (cucumber) and Lycopersicum esculentum (tomato) was done. The in vitro studies on A. cepa have been done to check the cytotoxicological effects including mitotic index, chromosomal aberrations, vagrant chromosomes, sticky chromosomes, disturbed metaphase, breaks and formation of micronucleus. In vitro and in vivo studies on seed systems exposed to different concentration of nanoparticles dispersion to check phytotoxicity end point as root length, germination effect, adsorption and accumulation of nanoparticles (uptake studies) into the plant systems. In vivo studies in a seed system was done using phytagel medium. Biochemical studies were done to check effect on protein, DNA and thiobarbituric acid reactive species concentration. FT-IR studies were done to analyze the functional and conformational changes in the treated and untreated samples. The toxicological effects of nanoparticles had to be studied at the miniscule scale to address existing environment problems or prevent future problems. The findings suggest that the engineered nanoparticles, though having significant advantages in research and medical applications, requires a great deal of toxicity database to ascertain the biosafety and risk of using engineered nanoparticles in consumer products.
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Osterberg R, Persson D, Bjursell G (1984) The condensation of DNA by chromium (III) ions. J Biomol Struct Dyn 2:285–290
The Royal Society and Royal Academy of Engineering, UK (2004) Nanoscience and nanotechnology, opportunities and uncertainties. Available at http://www.nanotech.org.uk/finalReport.htm
Munzuroglu O, Geckil H (2002) Effects of metals on seed germination, root elongation, and coleoptile and hypocotyl growth in Triticum aestivum and Cucumis sativus. Arch Environ Contam Toxicol 43:203–213
Oberdorster G, Oberdorster E, Oberdorster J (2005) Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ Health Perspect 113:823–839
Anastasio C, Martin ST (2001) Atmospheric nanoparticles. Rev Miner Geochem 44:293–349
Nel A, Xia T, Madler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311:622–627
Agency for toxic substances and Disease Registry (1990) Toxicological profile for silver prepared by clement international corporation under contract 205-88-0608, U.S. Public Health Service. ATSDR/TP-90-24
Maynard AD, Aitken RJ, Butz T, Colvin V, Donaldson K, Oberdörster G, Philbert MA, Ryan J, Seaton A, Stone V, Tinkle SS, Tran L, Walker NJ, Warheit DB (2006) Safe handling of nanotechnology. Nature 444:267–269
Chen X, Schluesener HJ (2008) Nanosilver: a nanoproduct in medical application. Toxicol Lett 176(Pt 1):1–12
Tripathy A, Chandrasekran N, Raichur AM, Mukherjee A (2008) Antibacterial applications of silver nanoparticles synthesized by aqueous extract of Azadirachta indica (Neem) leaves. J Biomed Nanotechnol 4:1–6
USEPA (2009) European Agency for Safety and Health Report
Wiesner MR, Lowry GV, Alvarez P, Dionisiou D, Biswas P (2006) Assessing the risks of manufactured nanomaterials. Environ Sci Technol 15:4336–4345
Xia T, Kovochich M, Brant J, Hotze M, Sempf J, Oberley T, Sioutas C, Yeh JI, Wiesner MR, Nel AE (2006) Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. Nano Lett 6:1794–1807
Hsin Y, Chen C, Huang S, Shih T, Lai P, Chueh PJ (2008) The apoptotic effect of nanosilver is mediated by a ROS- and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells. Toxicol Lett 179:130–139
Franklin NM, Rogers NJ, Apte SC, Batley E, Gadd E, Casey PS (2007) Comparative toxicity of nanoparticulate ZnO, bulk ZnO, and ZnCl to a freshwater microalga (Pseudokirchneriella subcapitata): the importance of particle solubility. Environ Sci Technol 41:8484–8490
USEPA (2007) Nanotechnology White Paper. Science Policy Council, Washington
Bakare AA, Mosuro AA, Osibanjo O (2000) Effect of simulated leachate on chromosomes and mitosis in roots of Allium cepa (L). J Environ Biol 21:263
U.S. Environmental Protection Agency Ecological Effects Test Guidelines (OPPTS 850.4200), Seed Germination/Root Elongation Toxicity Test (1996). http://www.epa.gov/opptsfrs/publications/OPPTS_Harmonized/850_Ecological_Effects_Test_Guidelines/Drafts/850-4200.pdf
Leme DM, Marin-Morales MA (2008) Chromosome aberration and micronucleus frequencies in Allium cepa cells exposed to petroleum polluted water—a case study. Mutat Res 650:80–86
USEPA Data Quality Objectives Decision Error Feasibility Trials (DQO/DEFT), Version 4.0, EPA QA/G-4D (1994) Washington
Lowry OH, Rosbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:267–275
Hoisington D, Khairallah M, Gonzales de leon D (1994) Laboratory protocols. CIMMYT Applied Biotechnology Center, Mexico
Zhu H, Han J, Xiao JQ, Jin Y (2008) Uptake, translocation and accumulation of manufactured iron oxide nanoparticles by pumpkin plants. J Environ Monit 10:713–717
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Kumari, M., Ernest, V., Mukherjee, A., Chandrasekaran, N. (2012). In Vivo Nanotoxicity Assays in Plant Models. In: Reineke, J. (eds) Nanotoxicity. Methods in Molecular Biology, vol 926. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-002-1_26
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DOI: https://doi.org/10.1007/978-1-62703-002-1_26
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