Abstract
We describe a method for highly sensitive high-throughput analysis of six major phytohormones; cytokinins (23 species), auxins (7 species), abscisic acid (ABA), gibberellins (GAs, 11 species), salicylic acid (SA), and jasmonic acid (JA). The method consists of solid-phase extraction using 96-well column plates and liquid chromatography coupled with a tandem quadrupole mass spectrometer. In order to improve the quantification limit of negatively charged compounds and especially GAs, we use a chemical modification with an “MS-probe” that contains a quaternary amine moiety. The method requires plant tissue of <100 mg fresh weight for quantification and enables us to analyze large number of plant samples at a time.
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References
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Kojima, M., Sakakibara, H. (2012). Highly Sensitive High-Throughput Profiling of Six Phytohormones Using MS-Probe Modification and Liquid Chromatography–Tandem Mass Spectrometry. In: Normanly, J. (eds) High-Throughput Phenotyping in Plants. Methods in Molecular Biology, vol 918. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-995-2_11
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DOI: https://doi.org/10.1007/978-1-61779-995-2_11
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-994-5
Online ISBN: 978-1-61779-995-2
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