Abstract
Antibody-based detection of protein distribution patterns both in wholemount and on sections revolutionized Xenopus research and ushered in the visual-based era of Xenopus data presentation. The ability to view the distribution of a gene product throughout an embryo makes it possible to rapidly map normal expression profiles and profiles that have been altered by an experimental intervention. The main limiting element in Xenopus immunostaining techniques has always been the availability of antibodies that work well on fixed whole embryos, a problem that persists. However, new antibodies are constantly being generated and improvements in detection systems allow antibodies that were once below the limits of detection to be utilized in multichannel immunofluorescence using tyramide amplification.
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Acknowledgements
This work was supported by the Wellcome Trust to NP and NIH R01 HD45776 and P41 HD064556 to PDV. The authors would like to thank Xiaolan Zhou for his role in developing tyramide protocols.
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Dubaissi, E., Panagiotaki, N., Papalopulu, N., Vize, P.D. (2012). Antibody Development and Use in Chromogenic and Fluorescent Immunostaining. In: HOPPLER, S., Vize, P. (eds) Xenopus Protocols. Methods in Molecular Biology, vol 917. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-992-1_23
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DOI: https://doi.org/10.1007/978-1-61779-992-1_23
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