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Immunoisolation of Protein Complexes from Xenopus

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Xenopus Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 917))

Abstract

The immunoaffinity isolation of protein complexes is an essential technique for the purification and ­concentration of protein complexes from cells and tissues. In this chapter we present the methodologies for the purification of proteins and protein complexes from Xenopus laevis and Xenopus tropicalis. Specific to this protocol are the techniques for the cryolysis of Xenopus cells and tissues, a procedure that limits contamination from yolk proteins while preserving endogenous protein complexes, the methodologies for immunoaffinity purification of proteins using magnetic beads, and the protocols for western blot analysis. In addition, the procedures in this chapter can be extended to use with proteomic analysis of protein complexes as presented in the following chapter.

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References

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Author information

Authors and Affiliations

  1. Department of Genetics, University of North Carolina, Chapel Hill, NC, USA

    Frank L. Conlon, Erin Kaltenbrun & Lauren Waldron

  2. Department of Molecular Biology, Princeton University, Princeton, NJ, USA

    Yana Miteva, Todd M. Greco & Ileana M. Cristea

Authors
  1. Frank L. Conlon
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  2. Yana Miteva
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  3. Erin Kaltenbrun
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  4. Lauren Waldron
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  5. Todd M. Greco
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  6. Ileana M. Cristea
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Corresponding authors

Correspondence to Frank L. Conlon or Ileana M. Cristea .

Editor information

Editors and Affiliations

  1. , Institute of Medical Sciences, University of Aberdeen, FORESTERHILL, ABERDEEN, AB25 2ZD, United Kingdom

    STEFAN HOPPLER

  2. , Department of Biological Science, University of Calgary, University Drive NW 2500, Calgary, T2N 1N4, Canada

    Peter D Vize

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© 2012 Springer Science+Business Media, LLC

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Conlon, F.L., Miteva, Y., Kaltenbrun, E., Waldron, L., Greco, T.M., Cristea, I.M. (2012). Immunoisolation of Protein Complexes from Xenopus . In: HOPPLER, S., Vize, P. (eds) Xenopus Protocols. Methods in Molecular Biology, vol 917. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-992-1_21

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  • DOI: https://doi.org/10.1007/978-1-61779-992-1_21

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-991-4

  • Online ISBN: 978-1-61779-992-1

  • eBook Packages: Springer Protocols

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