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Xenopus Protocols pp 231–243Cite as

Xenopus Transgenics: Methods Using Transposons

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 917))

Abstract

The generation of transgenic animals is an essential tool for many genetic strategies. DNA “cut-and-paste” transposon systems can be used to efficiently modify the Xenopus genome. The DNA transposon substrate, harbored on a circularized plasmid, is co-injected into fertilized Xenopus embryos at the one-cell stage together with mRNA encoding the cognate transposase enzyme. The cellular machinery rapidly translates the exogenous mRNA to produce active transposase enzyme that catalyzes excision of the transposon substrate from the plasmid and stable integration into the genomic DNA.

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Acknowledgments

This work was supported by NIH grants HD042994 and MH079381 and by the American Lebanese and Syrian Associated Charities (ALSAC) to PEM.

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Authors and Affiliations

  1. Department of Pathology, St. Jude Children’s Research Hospital, Memphis, TN, USA

    Clair M. Kelley, Donald A. Yergeau, Haiqing Zhu, Emin Kuliyev & Paul E. Mead

Authors
  1. Clair M. Kelley
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  2. Donald A. Yergeau
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  3. Haiqing Zhu
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  4. Emin Kuliyev
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  5. Paul E. Mead
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Corresponding author

Correspondence to Paul E. Mead .

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Editors and Affiliations

  1. , Institute of Medical Sciences, University of Aberdeen, FORESTERHILL, ABERDEEN, AB25 2ZD, United Kingdom

    STEFAN HOPPLER

  2. , Department of Biological Science, University of Calgary, University Drive NW 2500, Calgary, T2N 1N4, Canada

    Peter D Vize

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Kelley, C.M., Yergeau, D.A., Zhu, H., Kuliyev, E., Mead, P.E. (2012). Xenopus Transgenics: Methods Using Transposons. In: HOPPLER, S., Vize, P. (eds) Xenopus Protocols. Methods in Molecular Biology, vol 917. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-992-1_14

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  • DOI: https://doi.org/10.1007/978-1-61779-992-1_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-991-4

  • Online ISBN: 978-1-61779-992-1

  • eBook Packages: Springer Protocols

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