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Isotope Techniques to Study Kinetics of Na+ and K+ Transport Under Salinity Conditions

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Book cover Plant Salt Tolerance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 913))

Abstract

Radioisotopes (particularly 22Na, 24Na, 42K, and 86Rb) have been used for many decades to trace the fluxes and accumulation of sodium and potassium ions in plant tissues. In this article, standard procedures for the tracing of ion fluxes are described, with emphasis on special problems encountered when examining K+ and Na+ transport under salinity conditions. We focus in particular on unidirectional influx measurements, while also providing a brief introduction to compartmental analysis by tracer efflux.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Toronto, Toronto, ON, Canada

    Dev T. Britto & Herbert J. Kronzucker

Authors
  1. Dev T. Britto
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  2. Herbert J. Kronzucker
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Corresponding author

Correspondence to Herbert J. Kronzucker .

Editor information

Editors and Affiliations

  1. School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, 7001, Tasmania, Australia

    Sergey Shabala

  2. INRA-SupAgro, BPMP-IBIPGC, place Viala 1, Montpellier, 34060, France

    Tracey Ann Cuin

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Britto, D.T., Kronzucker, H.J. (2012). Isotope Techniques to Study Kinetics of Na+ and K+ Transport Under Salinity Conditions. In: Shabala, S., Cuin, T. (eds) Plant Salt Tolerance. Methods in Molecular Biology, vol 913. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-986-0_26

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  • DOI: https://doi.org/10.1007/978-1-61779-986-0_26

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-985-3

  • Online ISBN: 978-1-61779-986-0

  • eBook Packages: Springer Protocols

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