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Quantification of the Antioxidant Activity in Salt-Stressed Tissues

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Plant Salt Tolerance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 913))

Abstract

Biochemical methods available for the measurement of antioxidant activity in salt-stressed tissues are reviewed, outlining the most important advantages and shortcomings of the methods. Here we consider commonly used methods for measuring total antioxidant capacity and phenolic content, ABTS and Folin-Ciocalteu’s procedure, respectively. Moreover, we presented assays for determination of antioxidant enzymes activities: superoxide dismutase, catalase, and ascorbate peroxidase. This choice of methods enables us to elucidate a full profile of antioxidant activities, evaluating their effectiveness against various reactive oxygen species produced during salt stress.

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Acknowledgment

This work was supported by the Serbian Ministry of Education and Science (grants 173028 and 173040).

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Authors and Affiliations

  1. Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia

    Jelena J. Dragišić Maksimović & Branka D. Živanović

Authors
  1. Jelena J. Dragišić Maksimović
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  2. Branka D. Živanović
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Corresponding author

Correspondence to Jelena J. Dragišić Maksimović .

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Editors and Affiliations

  1. School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, 7001, Tasmania, Australia

    Sergey Shabala

  2. INRA-SupAgro, BPMP-IBIPGC, place Viala 1, Montpellier, 34060, France

    Tracey Ann Cuin

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Maksimović, J.J.D., Živanović, B.D. (2012). Quantification of the Antioxidant Activity in Salt-Stressed Tissues. In: Shabala, S., Cuin, T. (eds) Plant Salt Tolerance. Methods in Molecular Biology, vol 913. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-986-0_16

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  • DOI: https://doi.org/10.1007/978-1-61779-986-0_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-985-3

  • Online ISBN: 978-1-61779-986-0

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