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Plant Salt Tolerance pp 225–236Cite as

Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 913))

Abstract

Reactive oxygen species (ROS) participate in signaling events that regulate ion channel activity and gene expression. However, excess ROS exert adverse effects that stem from their interaction with macromolecules. Thus, the assessment of the effects of salinity on ROS changes are central to understanding how plants respond and cope with this stress. ROS determination in salt-stressed plants poses specific challenges. On the one hand, salinity comprises osmotic and ion-specific effects which may, in turn, have different effects on ROS production. On the other hand, changes in ROS production may happen when tissues from salinized plants are subject to water potential (Ψ) changes when incubated in non-isosmotic solutions. This chapter provides detailed accounts of methods for ROS detection in tissues from salt-stressed plants and includes suggestions for avoiding artifacts when dealing with such tissues.

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Acknowledgments

The authors gratefully acknowledge support from Agencia Nacional de Promoción Científica y Tecnológica (ANPCYT), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto Nacional de Tecnología Agropecuaria (INTA), and Universidad de San Martin (UNSAM), all from Argentina.

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Authors and Affiliations

  1. INTECH-CONICET, Chascomús, Argentina

    Andrés Alberto Rodríguez

  2. CIAP-INTA, Córdoba, Argentina

    Edith L. Taleisnik

Authors
  1. Andrés Alberto Rodríguez
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  2. Edith L. Taleisnik
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Corresponding author

Correspondence to Edith L. Taleisnik .

Editor information

Editors and Affiliations

  1. School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, 7001, Tasmania, Australia

    Sergey Shabala

  2. INRA-SupAgro, BPMP-IBIPGC, place Viala 1, Montpellier, 34060, France

    Tracey Ann Cuin

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Rodríguez, A.A., Taleisnik, E.L. (2012). Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues. In: Shabala, S., Cuin, T. (eds) Plant Salt Tolerance. Methods in Molecular Biology, vol 913. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-986-0_15

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  • DOI: https://doi.org/10.1007/978-1-61779-986-0_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-985-3

  • Online ISBN: 978-1-61779-986-0

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