Abstract
Both nitric oxide (NO) and reactive oxygen species (ROS) are versatile molecules that mediate a variety of cellular responses in plants. In this chapter, methods for imaging NO and ROS using laser scanning confocal microscopy (LSCM) are presented. Arabidopsis roots, dyed with DAF-FM or H2DCF, are observed using the Leica TCS-SP2 LSCM. NO or ROS production are imaged and their kinetic changes monitored with the laser excitation and emission wavelengths at 488 nm and between 500 and 530 nm, respectively. In addition, Leica software is employed to visualize and calculate the fluorescence intensity data.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (grant nos 31170241, 30971711 and 30671248), the Natural Science Foundation of Jiangsu Province of China (grant no. BK2009309), Fundamental Research Funds for the Central Universities (grants nos KYJ200912 and KYZ200905), the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the Technology Support Program in Jiangsu Province (grant no. BE2010382).
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Xie, YJ., Shen, WB. (2012). In vivo Imaging of Nitric Oxide and Reactive Oxygen Species Using Laser Scanning Confocal Microscopy. In: Shabala, S., Cuin, T. (eds) Plant Salt Tolerance. Methods in Molecular Biology, vol 913. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-986-0_12
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DOI: https://doi.org/10.1007/978-1-61779-986-0_12
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