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A Preliminary Assessment of Using a White Light Confocal Imaging Profiler for Cut Mark Analysis

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Forensic Microscopy for Skeletal Tissues

Part of the book series: Methods in Molecular Biology ((MIMB,volume 915))

Abstract

White light confocal microscopy creates detailed 3D representations of microsurfaces that can be qualitatively and quantitatively analyzed. The study describes its application to the analysis of cut marks on bone, particularly when discerning cuts made by steel tools from those made by stone. The process described comes from a study where cuts were manually made on a cow rib with seven cutting tools, four stone (an unmodified chert flake, a chert biface, a bifacially ground slate fragment, and an unsharpened piece of slate), and three steel (a Swiss Army Knife, a serrate steak knife, and a serrate saw). Kerfs were magnified ×20 and 3D data clouds were generated using a Sensofar® White Light Confocal Profiler (WLCP). Kerf profiles and surface areas, volumes, mean depths, and maximum depths were calculated with proprietary software (SensoScan® and SolarMap®). For the most part, the stone tools make shallower and wider cuts. Kerf floors can be studied at higher magnifications; they were viewed at ×100. When comparing the kerf floors of the unsharpened slate and the serrate steak knife it was found that the slate floor was more uneven, but the serrate steak knife generated more overall relief. Although preliminary, the approach described here successfully distinguishes stone and steel tools; the authors conclude that the WLCP is a promising technology for cut mark analysis because of the very detailed 3D representations it creates and the numerous avenues of analysis it provides.

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Acknowledgments

Thank you to Molly Hill Schmidt for editorial comments and to Lynne Bell for her patience and support for this chapter. Funding was provided by the National Science Foundation (to CWS), BCS-0922930.

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Correspondence to Christopher W. Schmidt .

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© 2012 Springer Science+Business Media, LLC

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Schmidt, C.W., Moore, C.R., Leifheit, R. (2012). A Preliminary Assessment of Using a White Light Confocal Imaging Profiler for Cut Mark Analysis. In: Bell, L. (eds) Forensic Microscopy for Skeletal Tissues. Methods in Molecular Biology, vol 915. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-977-8_14

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  • DOI: https://doi.org/10.1007/978-1-61779-977-8_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-976-1

  • Online ISBN: 978-1-61779-977-8

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