Abstract
One of the major bottlenecks in antibody discovery for research and therapeutic applications is the need for large quantities of protein in a short amount of time. Here we describe an alternative method using the Drosophila melanogaster S2 expression system to produce high levels of antibodies (both IgG and Fab) with equivalent binding properties to antibodies produced in mammalian cell expression systems. Using the Drosophila S2 expression system for antibody production has many advantages over current mammalian systems making antibody expression, purification, and evaluation a much less time-consuming process.
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Acknowledgements
We thank Mats A.A. Persson and Katarina Drakenberg for setting up the Drosophila S2 expression system and for critical reading of the manuscript. This project was supported by the Swedish Foundation for Strategic Research (Cell factory program).
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Johansson, D.X., Krey, T., Andersson, O. (2012). Production of Recombinant Antibodies in Drosophila melanogaster S2 Cells. In: Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 907. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-974-7_21
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DOI: https://doi.org/10.1007/978-1-61779-974-7_21
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61779-974-7
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