Abstract
Guided selection provides a powerful tool for humanization of the preexisting nonhuman antibodies as exemplified by HUMIRA, the world’s first human antibody approved. This chapter describes the sequential guided selection procedure in which mouse VL and VH domains are replaced sequentially with human VL and VH, respectively to derive completely human antibody. The detailed protocols for construction of phage-displayed antibody library, panning, screening, and characterization, are included to achieve successful selection of human antibody with similar characteristics to original mouse antibody.
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Acknowledgements
This work was supported by a Medicinal Bioconvergence Research Center grant from the Ministry of Science and Technology of Korea.
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Kim, S.J., Hong, H.J. (2012). Humanization by Guided Selections. In: Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 907. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-974-7_14
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DOI: https://doi.org/10.1007/978-1-61779-974-7_14
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Publisher Name: Humana Press, Totowa, NJ
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