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Utility of Animal Models for Human Embryo Culture Development: Rodents

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Embryo Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 912))

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Abstract

Advancements in clinical human embryo culture over the last 30-40 years have been supported by research conducted with embryos from rodent and domestic species. The mouse has been the primary rodent species that has contributed to improved embryo culture outcomes. Numerous parameters applied in the beginning of experiments, during progress of experiments, and as end-point measures provide varying degrees of rigor and interpretive strength and/or complexity. A nonexhaustive discussion of these parameters is presented with important emphasis on experimental design to obtain the greatest power of intraexperimental interpretation of inferior, equivalent, or improved culture conditions in the mouse model. Additionally, data are presented demonstrating the inherent flaw of overinterpretation of interexperimental outcome comparisons and caution of expectations of data translation from the mouse to the human embryo culture scenario. Finally, a materials, methods, and notes discussion enumerates important steps in use of mouse embryos as a bioassay tool, independent of whether they are being used in an experiment focused on quality control or improving culture conditions.

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Author information

Authors and Affiliations

  1. University of Michigan, Ann Arbor, MI, USA

    Gary D. Smith

Authors
  1. Gary D. Smith
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Correspondence to Gary D. Smith .

Editor information

Editors and Affiliations

  1. , OB/GYN, University of Michigan, E. Catherine Street 1137, Ann Arbor, 48109-5622, Michigan, USA

    Gary D. Smith

  2. , Center for Reproductive Medicine, University of Michigan, Market Street 475, Ann Arbor, 48108, Michigan, USA

    Jason E. Swain

  3. Fertility Center of San Antonio, Medical Drive 4499, San Antonio, 78229, Texas, USA

    Thomas B. Pool

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Smith, G.D. (2012). Utility of Animal Models for Human Embryo Culture Development: Rodents. In: Smith, G., Swain, J., Pool, T. (eds) Embryo Culture. Methods in Molecular Biology, vol 912. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-971-6_2

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  • DOI: https://doi.org/10.1007/978-1-61779-971-6_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-970-9

  • Online ISBN: 978-1-61779-971-6

  • eBook Packages: Springer Protocols

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