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Culture Systems: Low-Oxygen Culture

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Embryo Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 912))

Abstract

The tension of oxygen measured in the oviducts of several mammals was 5–8.7 %, but this drops in the uterine milieu to <2 % in cows and primates. For embryo culture in human in vitro fertilization (IVF), a non-physiologic level of 20 % oxygen has been used for the past 30 years. However, several animal studies have shown that low levels of oxygen plays an important physiological role in reducing the high levels of detrimental reactive oxygen species within cells, influences the embryonic gene expression, helps with embryo metabolism of glucose, and enhances embryo development to blastocysts. However, clinical studies have given contradictory results. Nevertheless, in nearly all reports, some kind of improvement has been observed, either in embryo development or in implantation and no detriments have been reported. For these reasons, more and more IVF laboratories utilize low oxygen during embryo culture.

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  1. Department of Human Reproduction and Gynecologic Endocrinology, University Medical Centre Maribor, Maribor, Slovenia

    Borut Kovačič

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  1. Borut Kovačič
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Correspondence to Borut Kovačič .

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Editors and Affiliations

  1. , OB/GYN, University of Michigan, E. Catherine Street 1137, Ann Arbor, 48109-5622, Michigan, USA

    Gary D. Smith

  2. , Center for Reproductive Medicine, University of Michigan, Market Street 475, Ann Arbor, 48108, Michigan, USA

    Jason E. Swain

  3. Fertility Center of San Antonio, Medical Drive 4499, San Antonio, 78229, Texas, USA

    Thomas B. Pool

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Kovačič, B. (2012). Culture Systems: Low-Oxygen Culture. In: Smith, G., Swain, J., Pool, T. (eds) Embryo Culture. Methods in Molecular Biology, vol 912. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-971-6_15

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  • DOI: https://doi.org/10.1007/978-1-61779-971-6_15

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