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Media Composition: pH and Buffers

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Embryo Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 912))

Abstract

The proper pH of media is a crucial parameter for optimizing efficacy of gamete and embryo culture. Selecting the right media pH and stabilizing this pH are important variables in minimizing intracellular stress and optimizing development. Regulation of intracellular (pHi) and extracellular pH (pHe) is discussed, as well as methods to prevent harmful pHe oscillations. Furthermore, proper approaches to ensure accurate measurement of media pHe are described.

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Author information

Authors and Affiliations

  1. University of Michigan, Ann Arbor, MI, USA

    Jason E. Swain

Authors
  1. Jason E. Swain
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Corresponding author

Correspondence to Jason E. Swain .

Editor information

Editors and Affiliations

  1. , OB/GYN, University of Michigan, E. Catherine Street 1137, Ann Arbor, 48109-5622, Michigan, USA

    Gary D. Smith

  2. , Center for Reproductive Medicine, University of Michigan, Market Street 475, Ann Arbor, 48108, Michigan, USA

    Jason E. Swain

  3. Fertility Center of San Antonio, Medical Drive 4499, San Antonio, 78229, Texas, USA

    Thomas B. Pool

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© 2012 Springer Science+Business Media New York

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Swain, J.E. (2012). Media Composition: pH and Buffers. In: Smith, G., Swain, J., Pool, T. (eds) Embryo Culture. Methods in Molecular Biology, vol 912. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-971-6_10

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  • DOI: https://doi.org/10.1007/978-1-61779-971-6_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-970-9

  • Online ISBN: 978-1-61779-971-6

  • eBook Packages: Springer Protocols

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